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Annadana, S.; Mlynárová, Ľudmila; Udayakumar, M.; Jong, Jan de; Nap, Jan-Peter

doi:10.1023/a:1015212312928

Cited by 26 publications

(2 citation statements)

References 37 publications

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“…Our findings demonstrated that the StLhca3 promoter is the best candidate to achieve robust expression in potato leaves. There were no comparative data for its functional activity in potato; however, it has been reported to provide much better gusA expression than CaMV 35S promoter in chrysanthemum [42] and tobacco [41]. The data from the present study support this tendency, as the constitutive expression of StUbi and CaMV 35S constructs in potato leaves did not exceed that of the StLhca3 promoter-gusA fusion.…”

Section: Discussionsupporting

confidence: 73%

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Miroshnichenko

Фирсов

Timerbaev

et al. 2020

Plants

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Various plant-derived promoters can be used to regulate ectopic gene expression in potato. In the present study, four promoters derived from the potato genome have been characterized by the expression of identical cassettes carrying the fusion with the reporter β-glucuronidase (gusA) gene. The strengths of StUbi, StGBSS, StPat, and StLhca3 promoters were compared with the conventional constitutive CaMV 35S promoter in various organs (leaves, stems, roots, and tubers) of greenhouse-grown plants. The final amount of gene product was determined at the post-transcriptional level using histochemical analysis, fluorometric measurements, and Western blot analysis. The promoter strength comparison demonstrated that the StUbi promoter generally provided a higher level of constitutive β-glucuronidase accumulation than the viral CaMV 35S promoter. Although the StLhca3 promoter was predominantly expressed in a green tissue-specific manner (leaves and stems) while StGBSS and StPat mainly provided tuber-specific activity, a “promoter leakage” was also found. However, the degree of unspecific activity depended on the particular transgenic line and tissue. According to fluorometric data, the functional activity of promoters in leaves could be arranged as follows: StLhca3 > StUbi > CaMV 35S > StPat > StGBSS (from highest to lowest). In tubers, the higher expression was detected in transgenic plants expressing StPat-gusA fusion construct, and the strength order was as follows: StPat > StGBSS > StUbi > CaMV 35S > StLhca3. The observed differences between expression patterns are discussed considering the benefits and limitations for the usage of each promoter to regulate the expression of genes in a particular potato tissue.

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Section: Discussionsupporting

confidence: 73%

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Miroshnichenko

Фирсов

Timerbaev

et al. 2020

Plants

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“…However, despite the success in the use of this promoter, some studies described expression patterns different from that expected for a constitutive promoter. In the case of the Asteraceae family, the lack of β-glucuronidase activity under the regulation of this promoter was described in chrysanthemum (Annadana et al, 2001;Outchkourov et al, 2003). These difficulties led to the use of new alternative promoters to express genes in both leaf and flower (Annadana et al, 2002a,b;Outchkourov et al, 2003;Aida et al, 2004Aida et al, , 2005.…”

Section: Sunflowermentioning

confidence: 99%

Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

et al. 2021

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The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.

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Matrix Attachment Regions and Transcriptional Gene Silencing

Thompson¹,

Spiker²,

Allen³

Regulation of Transcription in Plants

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Untitled

Cited by 26 publications

References 37 publications

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

Matrix Attachment Regions and Transcriptional Gene Silencing

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