Untitled

Abstract

A simple, accurate, and precise dual-wavelength spectrophotometric method was developed to determine Paracetamol and Piroxicam in combined pharmaceutical dosage form simultaneously. The principle for the dual wavelength method is "the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest". The pre-requisite for the dual wavelength method is the selection of two such wavelengths where the interfering component shows the same absorbance. In contrast, the component of interest shows a significant difference in absorbance with concentration. The wavelengths selected for paracetamol were 257nm, whereas the wavelengths selected for the determination of Piroxicam were 353nm. 0.5M NaOH was taken as a solvent. The regression analysis of Beer's plots showed good correlation in a concentration range of 2-10 μg/ml for paracetamol and 2-10 μg/ml for Piroxicam. The accuracy method was found to be between 97.33-101.5%. The method's precision (intra-day, inter-day, and repeatability) was found within limits. The proposed method was successfully applied to determine these drugs in commercial tablets.