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Abstract

We studied the state of lysosomal apparatus and pro- and antioxidant activity in the liver of rats with different resistance to hypoxia during postischemic recovery. Under normal conditions the lysosomal apparatus did not differ in highly and low resistant animals. During ischemia and reperfusion the damage to hepatic lysosomal membranes in rats highly resistant to hypoxia was less pronounced than in low resistant animals. These differences also concerned labilization of lysosomes during exposure to damaging factors (hypotonia and Triton X-100). The rats highly resistant to hypoxia differed from low resistant animals by higher stability of lysosomal membranes, lower prooxidant activity (malonic dialdehyde content), and higher tissue concentration of alpha-tocopherol during reperfusion.

Current strategies to minimize hepatic ischemia–reperfusion injury by targeting reactive oxygen species

“…More recent findings support these findings and documented that release of lysosomal iron and its uptake into mitochondria through the calcium uniporter can act synergistically with oxidant stress to promote the MPT and cell death [42]. Since hepatic ischemia and reperfusion can trigger lysosomal instability [63,64] and oxidant stress can be produced by mitochondria or extracellularly by leukocytes, which then triggers an intracellular stress, the combination of the lysosomal iron and ROS can induce the MPT and necrosis [42]. The critical importance of mitochondrial dysfunction and the MPT in determining cell death during hepatic warm and cold ischemia–reperfusion injury has been demonstrated by the protective effects of cyclophilin D inhibitors [61,62].…”

Photoperiod-induced alterations in biomarkers of oxidative stress in rats of different ages and individual physiological reactivity

“…Animals with high resistance (HR) to acute hypobaric hypoxia were identified among laboratory mice, rats, rabbits, and guinea pigs [37,43,44]. Intra-species differences in the reaction of individuals to acute hypoxia also vary significantly, and they are related to both genotypic and phenotypic characteristics of individuals [10,14].…”