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Gasperis, Giovanni De; Yang, Jun; Becker, Frederick F.; Gascoyne, Peter R. C.; Wang, Xiaobo

doi:10.1023/a:1009955200029

Cited by 43 publications

(24 citation statements)

References 23 publications

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“…DEP has also been combined with field flow fractionation [23] and travelling wave dielectrophoresis [24] for separating malaria infected cells [25] or cancer cells [26][27][28] from normal cells. We have used DEP in a microsystem for PCR sample preparation.…”

Section: Dielectrophoresis (Dep) Is An Electrokinetic Techniquementioning

confidence: 99%

“…This problem might be relieved by replacing the Ti/Au electrodes with metal-silicide electrodes [44]. Low-conductivity media have been used in the vast majority of dielectrophoretic cell sorting experiments reported in the literature [25][26][27][28][29]. Many cells (e.g., human or animal cells) require media with physiological osmotic pressure to avoid cell lysis.…”

Section: Dep Filter Characterizationmentioning

confidence: 99%

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Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

et al. 2006

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Dielectrophoresis (DEP) and flow cytometry are powerful technologies and widely applied in microfluidic systems for handling and measuring cells and particles. Here, we present a novel microchip with a DEP selective filter integrated with two microchip flow cytometers (FCs) for on-line monitoring of cell sorting processes. On the microchip, the DEP filter is integrated in a microfluidic channel network to sort yeast cells by positive DEP. The two FCs detection windows are set upstream and downstream of the DEP filter. When a cell passes through the detection windows, the light scattered by the cell is measured by integrated polymer optical elements (waveguide, lens, and fiber coupler). By comparing the cell counting rates measured by the two FCs, the collection efficiency of the DEP filter can be determined. The chips were used for quantitative determination of the effect of flow rate, applied voltage, conductivity of the sample, and frequency of the electric field on the sorting efficiency. A theoretical model for the capture efficiency was developed and a reasonable agreement with the experimental results observed. Viable and non-viable yeast cells showed different frequency dependencies and were sorted with high efficiency. At 2 MHz, more than 90% of the viable and less than 10% of the non-viable cells were captured on the DEP filter. The presented approach provides quantitative real-time data for sorting a large number of cells and will allow optimization of the conditions for, e.g., collecting cancer cells on a DEP filter while normal cells pass through the system. Furthermore, the microstructure is simple to fabricate and can easily be integrated with other microstructures for lab-on-a-chip applications.

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Section: Dielectrophoresis (Dep) Is An Electrokinetic Techniquementioning

confidence: 99%

Section: Dep Filter Characterizationmentioning

confidence: 99%

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

et al. 2006

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“…Since the ground breaking work of Pohl [1,2], dielectrophoresis has been extensively studied for the purpose of manipulation of synthetic [3][4][5][6][7] and biological particles [8][9][10][11], namely cells [2,4,10,[12][13][14][15][16][17][18], bacteria [19][20][21][22][23][24][25], viruses [26][27][28][29][30], and DNA [31][32][33][34]. Typical applications addressed so far include the development of methods for sorting and separation of particles [4,5,22,[35][36][37][38][39][40][41][42][43][44], ...…”

Section: Dielectrophoresismentioning

confidence: 99%

Numerical modelling and measurement of cell trajectories in 3‐D under the influence of dielectrophoretic and hydrodynamic forces

et al. 2011

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This research is part of a program aiming at the development of a fluidic microsystem for in vitro drug testing. For this purpose, primary cells need to be assembled to form cellular aggregates in such a way as to resemble the basic functional units of organs. By providing for in vivo-like cellular contacts, proper extracellular matrix interaction and medium perfusion it is expected that cells will retain their phenotype over prolonged periods of time. In this way, in vitro test systems exhibiting in vivo type predictivity in drug testing are envisioned. Towards this goal a 3-D microstructure micro-milled in a cyclic olefin copolymer (COC) was designed in such a way as to assemble liver cells via insulator-based dielectrophoresis (iDEP) in a sinusoid-type fashion. First, numeric modelling and simulation of dielectrophoretic and hydrodynamic forces acting on cells in this microsystem was performed. In particular, the problem of the discontinuity of the electric field at the interface between the fluid media in the system and the polymer materials it consists of was addressed. It was shown that in certain cases, the material of the microsystem may be neglected altogether without introducing considerable error into the numerical solution. This simplification enabled the simulation of 3-D cell trajectories in complex chip geometries. Secondly, the assembly of HepG2 cells by insulator-based dielectrophoresis in this device is demonstrated. Finally, theoretical results were validated by recording 3-D cell trajectories and the Clausius-Mossotti factor of liver cells was determined by combining results obtained from both simulation and experiment.

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“…DEP can move particles over distances from a few micrometers to several millimetres by the use of appropriately designed electrodes. Measurement of the DEP force as a function of frequency allows extraction of cellular electrophysiological data, and has been shown to be useful in a range of settings from analysis of cancer cells [1][2][3] to cellular apoptosis [4,5] and the action of drugs on bacteria [6,7].…”

Section: Introductionmentioning

confidence: 99%

Rapid, automated measurement of dielectrophoretic forces using DEP‐activated microwells

et al. 2011

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Dielectrophoresis (DEP) is a physical effect that generates a force on a polarisable particles experiencing a non-homogeneous electric field. It has been shown that this effect depends on the electrical properties of that particle, i.e. the electrical permittivity and conductivity. In the past, DEP-based techniques were applied to measure the electric properties of one or several cells at a time. The results showed that the measure of electrical properties by DEP is very sensitive to noise. However, further improvements are possible by generating more information from the experiments: this paper presents a rapid automated system that measures the DEP spectrum from a large population of cells with a low level of noise using the microwell electrodes, and a method of analysis that provides additional information about the electrical properties of the cells and a new theoretical approach was developed to obtain accurate, bias-free results in under five minutes.

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Cited by 43 publications

References 23 publications

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

Numerical modelling and measurement of cell trajectories in 3‐D under the influence of dielectrophoretic and hydrodynamic forces

Rapid, automated measurement of dielectrophoretic forces using DEP‐activated microwells

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