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Abstract

The human breast cancer cell line MCF7 does not express heart-type fatty acid binding protein (H-FABP), a marker protein for differentiated mammary gland. MCF7 cells transfected with the bovine H-FABP cDNA expressed the corresponding protein and were characterized by growth inhibition and lower tumorgenicity in nude mice [22]. By enzyme linked immunoassay we now determined the amount of bovine H-FABP in these cells as 638 +/- 80 ng/mg protein and used the transfected cells to study the role of H-FABP in fatty acid metabolism. Compared to control cells the uptake of radioactively labelled palmitic acid and oleic acid into MCF7 cells after 30 or 60 min was increased by 67% in H-FABP expressing transfectants, demonstrating a stimulatory role for this FABP-type in fatty acid metabolism. However, preferential targeting of [14C]oleic acid into neutral or phospholipid classes was not observed by the criterion of high performance thin layer chromatography followed by autoradiography. A reason for the modest increase of fatty acid uptake in H-FABP transfected MCF7 cells may be the basal expression of epidermal-type FABP, which was detected for the first time in these cells. It appears that the small amount of E-FABP expressed in MCF7 cells fulfils the need of the cells for a cytosolic fatty acid carrier under culture conditions and that even high concentrations of another FABP do only slightly increase the uptake due to limitations of fatty acid transport through the plasma membrane or of metabolism.

Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis

“…It is worth noting that the content of certain proteins (e.g. proteoglycans, elastic fibres-associated proteins [ 104 , 112 ], proteins involved in lipids metabolism [ [179] , [180] , [181] , [182] ]) was decreased in hDFs by + MMC treatment, whilst this effect was almost negligible in hTC cultures. Intriguingly, whilst under -MMC conditions, the content of these proteins was higher in hDFs than in hTCs, under + MMC conditions such differences no longer existed; MMC therefore may help balancing the levels of certain proteins between cultures of both cell types.…”

Challenges in enriching milk fat with polyunsaturated fatty acids

“…Overexpression bovine FABP3 in the non-secretory bovine mammary epithelial cell line, MAC-T, did not influence cellular fatty acid composition (Stamey and Corl, unpublished results). The human breast cancer cell line MCF7 does not express FABP3 and overexpression of FABP3 in MCF7 cells increased uptake of radioactively labeled palmitate and oleate, but other changes in fatty acid metabolism were not observed [ 79 ]. Kadegowda et al [ 80 ] reported FABP3 expression increased in MAC-T cells when treated with rosiglitazone to activate proliferator activated receptor-γ and palmitate or stearate.…”

LIBP-Pred: web server for lipid binding proteins using structural network parameters; PDB mining of human cancer biomarkers and drug targets in parasites and bacteria

“…Liver FABP (L-FABP) is a new prognostic factor for hepatic resection of colorectal cancer metastases, FABP-6 7 is also overexpressed in colorectal cancer, and the overexpression of FABP-7 8 correlates with basal-like subtype of breast cancer. There have been studies on the fatty acid metabolism in human breast cancer cells (MCF7) transfected with heart-type FABP (H-FABP) 9 and the role of sterol regulatory element binding protein-1c 10 in the regulation of fatty acid synthase expression in breast cancer. Li and Huang et al 11 carried out a prognostic evaluation of epidermal FABP and calcyphosine, two proteins implicated in endometrial cancer using a proteomic approach.…”