“…The protein yield per sugars consumed obtained, of 0.20 g g -1 , was similar to the reported value for shake flask cultures of C. blankii grown on xylose (0.22 g g −1 ) [6]. Carbohydrate content was higher than the values reported for C. langeronii [8], and K. fragilis [23], but in the range of C. blankii [6]. These values are typical of carbon excess growth conditions for this yeast [33], and hence they can be further improved, namely, the protein content.…”
“…The total protein content of 31.8% is compared to 31.3 % reported for C. tropicalis [7], 37% for Kluyveromyces fragilis [23], and 48.1% for C. langueronii [8]. The protein yield per sugars consumed obtained, of 0.20 g g -1 , was similar to the reported value for shake flask cultures of C. blankii grown on xylose (0.22 g g −1 ) [6].…”
“…It has approximately 26 g l −1 of monosaccharides and a low level of microbial inhibitors, specially aliphatic acids, and furan derivatives compared to similar hemicellulosic hydrolyzates used for SCP production, e.g., eucalyptus wood [28] and sugar cane bagasse [7,8]. Also, it has a low content of phenolic compounds.…”
Section: Feedstock and Hydrolyzate Compositionmentioning
confidence: 99%
“…Candida langeronii grown in sugar cane bagasse presented a higher biomass productivity of 0.97 g l −1 h −1 [8], but under fully controlled oxygen and pH conditions and using a higher sugar content. Nevertheless, the biomass yield for C. langeronii was 0.40 g g −1 , which is lower than that obtained for D. hansenii.…”
Section: Yeast Growth In Hemicellulosic Bsg Hydrolyzatementioning
confidence: 99%
“…The yeasts currently used and/or studied for SCP production, e.g., Candida utilis, are not able to utilize all sugars present, namely, arabinose, and as they belong to the Candida genus [6][7][8], they are not suitable for QPS status. It is thus important to develop new YE/ SCP producing processes based on different yeasts.…”
Yeast single-cell protein and yeast extract, in particular, are two products which have many feed, food, pharmaceutical, and biotechnological applications. However, many of these applications are limited by their market price. Specifically, the yeast extract requirements for culture media are one of the major technical hurdles to be overcome for the development of low-cost fermentation routes for several top value chemicals in a biorefinery framework. A potential biotechnical solution is the production of yeast biomass from the hemicellulosic fraction stream. The growth of three pentose-assimilating yeast cell factories, Debaryomyces hansenii, Kluyveromyces marxianus, and Pichia stipitis was compared using non-detoxified brewery's spent grains hemicellulosic hydrolyzate supplemented with mineral nutrients. The yeasts exhibited different specific growth rates, biomass productivities, and yields being D. hansenii as the yeast species that presented the best performance, assimilating all sugars and noteworthy consuming most of the hydrolyzate inhibitors. Under optimized conditions, D. hansenii displayed a maximum specific growth rate, biomass yield, and productivity of 0.34 h(-1), 0.61 g g(-1), and 0.56 g l(-1) h(-1), respectively. The nutritional profile of D. hansenii was thoroughly evaluated, and it compares favorably to others reported in literature. It contains considerable amounts of some essential amino acids and a high ratio of unsaturated over saturated fatty acids.
“…The protein yield per sugars consumed obtained, of 0.20 g g -1 , was similar to the reported value for shake flask cultures of C. blankii grown on xylose (0.22 g g −1 ) [6]. Carbohydrate content was higher than the values reported for C. langeronii [8], and K. fragilis [23], but in the range of C. blankii [6]. These values are typical of carbon excess growth conditions for this yeast [33], and hence they can be further improved, namely, the protein content.…”
“…The total protein content of 31.8% is compared to 31.3 % reported for C. tropicalis [7], 37% for Kluyveromyces fragilis [23], and 48.1% for C. langueronii [8]. The protein yield per sugars consumed obtained, of 0.20 g g -1 , was similar to the reported value for shake flask cultures of C. blankii grown on xylose (0.22 g g −1 ) [6].…”
“…It has approximately 26 g l −1 of monosaccharides and a low level of microbial inhibitors, specially aliphatic acids, and furan derivatives compared to similar hemicellulosic hydrolyzates used for SCP production, e.g., eucalyptus wood [28] and sugar cane bagasse [7,8]. Also, it has a low content of phenolic compounds.…”
Section: Feedstock and Hydrolyzate Compositionmentioning
confidence: 99%
“…Candida langeronii grown in sugar cane bagasse presented a higher biomass productivity of 0.97 g l −1 h −1 [8], but under fully controlled oxygen and pH conditions and using a higher sugar content. Nevertheless, the biomass yield for C. langeronii was 0.40 g g −1 , which is lower than that obtained for D. hansenii.…”
Section: Yeast Growth In Hemicellulosic Bsg Hydrolyzatementioning
confidence: 99%
“…The yeasts currently used and/or studied for SCP production, e.g., Candida utilis, are not able to utilize all sugars present, namely, arabinose, and as they belong to the Candida genus [6][7][8], they are not suitable for QPS status. It is thus important to develop new YE/ SCP producing processes based on different yeasts.…”
Yeast single-cell protein and yeast extract, in particular, are two products which have many feed, food, pharmaceutical, and biotechnological applications. However, many of these applications are limited by their market price. Specifically, the yeast extract requirements for culture media are one of the major technical hurdles to be overcome for the development of low-cost fermentation routes for several top value chemicals in a biorefinery framework. A potential biotechnical solution is the production of yeast biomass from the hemicellulosic fraction stream. The growth of three pentose-assimilating yeast cell factories, Debaryomyces hansenii, Kluyveromyces marxianus, and Pichia stipitis was compared using non-detoxified brewery's spent grains hemicellulosic hydrolyzate supplemented with mineral nutrients. The yeasts exhibited different specific growth rates, biomass productivities, and yields being D. hansenii as the yeast species that presented the best performance, assimilating all sugars and noteworthy consuming most of the hydrolyzate inhibitors. Under optimized conditions, D. hansenii displayed a maximum specific growth rate, biomass yield, and productivity of 0.34 h(-1), 0.61 g g(-1), and 0.56 g l(-1) h(-1), respectively. The nutritional profile of D. hansenii was thoroughly evaluated, and it compares favorably to others reported in literature. It contains considerable amounts of some essential amino acids and a high ratio of unsaturated over saturated fatty acids.
In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 20th Sept. 2000)
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