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Abstract

A system for rapid plant regeneration through somatic embryogenesis from shoot tip explants of sorghum [Sorghum bicolor (L.) Moench] is described. Somatic embryogenesis was observed after incubation of explants in dark for 6-7 weeks through a friable embryogenic callus phase. Linsmaier and Skoog medium supplemented with 2,4dichlorophenoxyacetic acid (2 mg l −1 ) and kinetin (0.1 mg l −1 ) was used for induction of friable embryogenic calli and somatic embryos. Germination of somatic embryos was achieved about 5 weeks after transfer onto Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (2 mg l −1 ) and indole-3-acetic acid (0.5 mg l −1 ) under light. Seeds from in vitro-regenerated plants produced a normal crop in a field trial, and were comparable to the crop grown with the seeds of the mother plant used to initiate tissue culture. The simplicity of the protocol and possible advantages of the system for transformation over other protocols using different explants are discussed.Abbreviations: 2,4-D -2,4 -dichlorophenoxy acetic acid; BA -6-benzylaminopurine; FEC -friable embryogenic callus; IAA -indoleacetic acid; MS -Murashige and Skoog; LS -Linsmaier and Skoog

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

“…In addition, a key aspect of the WU regeneration protocol was the use of ABA. A survey of various sorghum somatic embryogenesis studies [27,30,46,48,49,[52][53][54][55][56] indicated that the inclusion of auxin(s) and cytokinin(s) are standard for regeneration, but few use ABA for embryo maturation/ regeneration [49,53,55]. ABA is known to promote better embryogenic responses, with higher quality embryo formation, and subsequent plantlet conversion [57], so the superior performance of explants with the WU protocol may also reflect the inclusion of ABA during regeneration.…”

Sweet sorghum as biofuel feedstock: recent advances and available resources

“…The basal media that have been used in sweet sorghum callus induction and/or regeneration include Murashige and Skoog (MS), Linsmaier & Skoog (LS), and Gamborg. The most common formulation used for callus induction is MS basal medium with 2 mg/l 2,4- d , 0.5–1 mg/l kinetin, and 3% sucrose [135, 139, 141, 142]. Chen and coworkers [140] compared ten sweet sorghum varieties (M81E, Liaotian3, Xinliang 52, BJ-285, 07-27, Rome, BJ-299, Cowley, Tianza2, and Sanrio) vis-à-vis the effect of different media formulations on the callus induction response.…”