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Abstract

A hydrocarbon degrader isolated from a chronically oil-polluted marine site was identified as Rhodococcus sp. on the basis of morphology, fatty acid methyl ester pattern, cell wall analysis, biochemical tests and G + C content of DNA. It degraded up to 50% of the aliphatic fraction of Assam crude oil, in seawater supplemented with 35 mM nitrogen as urea and 0.1 mM phosphorus as dipotassium hydrogen orthophosphate, after 72 h at 30 degrees C and 150 revolutions per minute. The relative percentage of intracellular fatty acid was higher in hydrocarbon-grown cells compared to fructose-grown cells. The fatty acids C16, C16:1, C18 and C18:1 were constitutively present regardless of the growth substrate. In addition to these constitutive acids, other intracellular fatty acids varied in correlation to the hydrocarbon chain length supplied as a substrate. When grown on odd carbon number alkanes, the isolate released only monocarboxylic acids into the growth medium. On even carbon number alkanes only dicarboxylic acids were produced.

Genome analysis of deep sea piezotolerant Nesiotobacter exalbescens COD22 and toluene degradation studies under high pressure condition

“…Interestingly, the aromatic benzene compounds and its derivatives were effectively degraded in 120 h and elucidated by the accumulation of benzoic acid, 4-ethoxy at Rt (40.324) and 1, 2-benzenedicarboxylic acid at Rt (55.622), respectively. The accumulation of carboxylic acids corroborated with the research findings of Sharma and Pant (2000) 14 . At 10 MPa culture conditions, the major end product benzyl alcohol ( Rt 17.902) was observed within 72 h of incubation (Table 2).…”

Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria

“…They reported that the surfactin started to precipitate out of the production medium at pH 5 and was dissolved completely when the pH returned to 6.1. As a result, physical characterization revealed that the surfactin has excellent surface and emulsifying activities and it showed high stabilities over a wide pH range (5)(6)(7)(8)(9)(10). These properties make the surfactin biosurfactant potential candidate to be used in the bioremediation of contaminated sites and in the petroleum industry (MEOR) where drastic conditions commonly prevail.…”

“…Biosurfactants are widely used in different industries, such as cosmetics, special chemicals, food, pharmaceutics, agriculture, cleaners and microbial enhanced oil recovery (MEOR) [10][11][12][13]. The last mentioned application has attracted more attention because only 30% of oil present in reservoir can generally be recovered using primary and secondary recovery techniques [1].…”

Physiological and biochemical characterization and genome analysis of Rhodococcus qingshengii strain 7B capable of crude oil degradation and plant stimulation

“…In the genome of strain 7B, there are gene clusters of heterobactin and erythrochelin production, as well as individual elements related to the gene cluster of atratumycin production. Atratumycin is a cyclodepsipeptide with activity against Mycobacteria tuberculosis isolated from deep-sea derived Streptomyces atratus [53] . In turn, erythrochelin is a hydroxamate-type siderophore, the production of which is characteristic of Saccharopolyspora erythraea [54] .…”