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Goswami, M.; Shivaraman, N.; Singh, Raghuvir

doi:10.1023/a:1020429109524

Cited by 35 publications

(5 citation statements)

References 13 publications

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“…DP-4, Rhodococcus erythropolis, Burkholderia cepacia AC1100 (also known as Burkholderia phenoliruptrix AC1100), and Mycobacterium chlorophenolicum PCP-1. [9][10][11] Of all these bacteria, Burkholderia cepacia AC1100 has gained the most interest, partly due to its ability of using 2,4,5-trichlorophenoxyacetic acid as the sole carbon source, and partly due to its broad substrate specicity toward poly-CPs. [12][13][14][15] Burkholderia cepacia AC1100 can efficiently degrade many poly-CPs, such as 2,4,5-trichlorophenol (2,4,5-TCP), 2,4,6-trichlorophenol (2,4,6-TCP), 2,3,4,6-trichlorophenol (2,3,4,, and pentachlorophenol (PCP).…”

Section: Introductionmentioning

confidence: 99%

Insights into the catalytic mechanism of chlorophenol 4-monooxygenase: a quantum mechanics/molecular mechanics study

Zhang

et al. 2015

RSC Adv.

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The catalytic mechanism of chlorophenol 4-monooxygenase (TftD) toward substrates 2,4,5-trichlorophenol (2,4,5-TCP), 2,4,6-trichlorophenol (2,4,6-TCP), and 2,5-dichloro-p-hydroquinone (2,5-DiCHQ) was studied by quantum mechanical/molecular mechanical (QM/MM) investigations. The hydroxylation rather than dechlorination was found to be the rate determining step for all three substrates. Substantial energy barrier spreads on the hydroxylation steps have been found. The corresponding Boltzmann-weighted average barriers are 16.3, 17.8, and 19.7 kcal mol À1 for 2,4,5-TCP, 2,4,6-TCP, and 2,5-DiCHQ, in accordance with the experimentally determined reaction rates. A previously unnoticed residue Arg100 was found to be important during the hydroxylation steps.Electrostatic influences of ten active site residues on the hydroxylation step were also investigated.Glu251 and Arg366 were highlighted as candidate residues for future mutation studies. In addition, we have shown that spatial location of 2,6-DiCHQ as well as the hydroxylation barrier are the two possible reasons why 2,6-DiCHQ cannot be further degraded by TftD.

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Section: Introductionmentioning

confidence: 99%

Insights into the catalytic mechanism of chlorophenol 4-monooxygenase: a quantum mechanics/molecular mechanics study

Zhang

et al. 2015

RSC Adv.

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“…The column used was Agilent TC-C18 (25 mm × 4.6 mm); the sample was eluted at a flow rate of 0.75 mL/min with mobile phase consisting of methanol:water (80:20); detection wavelength is set to 280 nm (Sahinkaya and Dilek 2007; Karn et al 2010). 2-CP, 3-CP, and 4-CP were also analyzed using UV–Visible spectrophotometer at 273, 273 and 279 nm wavelengths, respectively (Goswami et al 2002). …”

Section: Methodsmentioning

confidence: 99%

Multi-substrate biodegradation of chlorophenols by defined microbial consortium

Patel

Kumar

2016

3 Biotech

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In the present study, a defined mixed microbial consortium was investigated for their ability to utilize three different monochlorophenols (MCPs) and 2,4-DCP individually and in the mixture. None of the individual strains were able to utilize 3-CP and 4-CP, but when they were mixed to form defined consortium, they have shown great potential and degradation of high concentration of 3-CP and 4-CP. Spectrophotometric analysis of metabolites during MCPs degradation establishes the presence of 2-chloromaleylacetate. Multi-substrate degradation study of 2,4-DCP in the presence of three MCPs showed the great prospect of microbial consortium for in situ bioremediation. During multi-substrate degradation, the biodegradation rate (mg L−1 day−1) was observed in the order of 2,4-DCP > 2CP > 3CP > 4CP. Biodegradation kinetic of three MCPs using Andrew’s model showed maximum removal rate (R m) of 2.78, 0.91, 1.82 mg L−1 h−1 for 2-CP, 3-CP and 4-CP, respectively.

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“…Two mesophilic (optimum growth temperature 28 • C), 2-CP-degrading strains of the genus Rhodococcus, R. opacus IG (DSM 43205) (Klatte et al, 1994) and R. erythropolis DSM 43066 (Goodfellow and Anderson, 1977), were used in this study as soil isolates previously evaluated for their ability to degrade 2-CP (Bondar et al, 1999;Goswami et al, 2002;Arora and Bae, 2014). These strains were grown in medium 535 (Klatte et al, 1994) as recommended by the DSMZ (German Collection of Microorganisms and Cell Cultures) which composition (per liter) included: pancreatic digest of casein (17.0 g), enzymatic digest of soy bean (3.0 g), sodium chloride (5.0 g), dipotasium hydrogen phosphate (2.5 g), and glucose (2.5 g).…”

Section: Bacterial Strains and Soil Samplesmentioning

confidence: 99%

“…The supernatant was collected and filtered through a 0.2 µm-pore-diameter filter. The extracted solution was injected in the described HPLC system and 2-CP quantified by peak integration after absorbance measurement at 273 nm (Goswami et al, 2002). 2-CP quantifications over time were used to estimate the decomposition rate (per hour) through the linear portion of the decay curve (Figure 1).…”

Section: Biodegradation Of 2-chlorophenolmentioning

confidence: 99%

Influence of Abiotic Factors Temperature and Water Content on Bacterial 2-Chlorophenol Biodegradation in Soils

Moxley

Puerta-Fernández

Gómez

et al. 2019

Front. Environ. Sci.

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Halogenated compounds are environmental pollutants toxic to humans and wildlife. Certain microorganisms degrade these halogenated compounds. However, little is known about the potential of microorganisms in bioremediation under extreme conditions, specifically in arid and semi-arid soils frequently exposed to high temperatures and desiccation periods. Arid and semi-arid environments and deserts make up vast areas of Earth's landmass. To investigate the degradation of 2-chlorophenol (2-CP) in soils as a function of temperature and water availability, three bacterial species were tested, two soil mesophiles of the genus Rhodococcus, R. opacus and R. erythropolis, and a soil thermophilic isolate, Parageobacillus thermoglucosidasius. Degradation trials in soil samples with these species were performed over a range of water activity from 1 to 0.4. At their optimum growth temperature, R. opacus showed maximum 2-CP degradation at water activity 0.9 sharply decreasing when lowering water activity. Nevertheless, the Parageobacillus isolate (optimum growth temperature 60 • C) showed maximum 2-CP degradation rates at water activity 0.5 which represented highly desiccating conditions. Parageobacillus degradation of 2-CP was very low at water activity above 0.9. Thus, biodegradation of 2-CP in soils is possible even under arid conditions although different microbial species might be involved in this task depending on the interactions of abiotic factors and the diversity of microbial communities in soils. These results contribute to understand the potential biodegradation of specific halogenated compounds in the environment which is of great relevance to comprehend the fate of halogenated pollutants (i.e., 2-CP) in deserts, arid and semi-arid soils.

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Cited by 35 publications

References 13 publications

Insights into the catalytic mechanism of chlorophenol 4-monooxygenase: a quantum mechanics/molecular mechanics study

Insights into the catalytic mechanism of chlorophenol 4-monooxygenase: a quantum mechanics/molecular mechanics study

Multi-substrate biodegradation of chlorophenols by defined microbial consortium

Influence of Abiotic Factors Temperature and Water Content on Bacterial 2-Chlorophenol Biodegradation in Soils

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