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Krasnov, E. A.; Ermilova, Elena; Kadyrova, T. V.; Ралдугин, В. А.; Bagryanskaya, I. Yu.; Gatilov, Yu. V.; Druganov, A. G.; Semenov, A. A.; Толстиков, Г. А.

doi:10.1023/a:1002887406817

Cited by 15 publications

(4 citation statements)

References 7 publications

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“…(C-7), 103.6 (C-6), 40.5 (C-2), 31.6 (C-1). Compared with the reported data, the 1 H NMR and 13 C NMR data are in agreement with that of 2′, 4′-dihydroxychalcone in the literature [22].…”

Section: Resultssupporting

confidence: 90%

Efficient separation of five flavonoids from Oxytropis falcata bunge by high-speed counter-current chromatography and their anticancer activity

Chen

Liu

Zhao

et al. 2020

Acta Chromatographica

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Oxytropis falcata Bunge, known as the “king of herbs” in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation, and anthrax. However, it is difficult to efficiently isolate compounds with high purity from O. falcata because of the complexity of traditional Tibetan medicines. In this study, the 80% ethanol elution fraction from extract by AB-8 macroporous resin column chromatography was demonstrated to have anticancer activity on human hepatoma SMMC-7721 cells in vitro. Then, a high-speed counter-current chromatography (HSCCC) method was successfully established for separation of compounds by using hexane–ethyl acetate–methanol–water (10:4:10:10, v/v/v/v) as the solvent system. Five flavonoids (7-hydroxyflavonone [1], 5,7-dihydroxy-4′-methoxy flavonol [2], 5,7-dihydroxyflavanone [3], 2′,4′-dihydroxychalcone [4], and 2′,4′-dihydroxydihydrochalcone [5]) were obtained in one-step separation with purities of 97.7%, 98.1%, 98.3%, 99.0%, and 98.3%, respectively. Finally, anticancer activities against the growth of SMMC-7721 cells of 5 flavonoids were confirmed. The IC50 values of the separated compounds were 213.45 μg/mL, 197.74 μg/mL, 375.16 μg/mL, 17.44 μg/mL, and 136.83 μg/mL in 24 h, respectively. The present study provided a basis for further development and utilization of this medicinal herb as a source of a new potential anticancer agent.

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Section: Resultssupporting

confidence: 90%

Efficient separation of five flavonoids from Oxytropis falcata bunge by high-speed counter-current chromatography and their anticancer activity

Chen

Liu

Zhao

et al. 2020

Acta Chromatographica

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“…Yield: 89.3%. NMR data for 3 was consistent with those previously reported [22]. and samples were taken at time intervals (6, 12, 24 and 48 h) and spun at 3500 rpm for 7 min in microcentrifugetubes.…”

Section: Synthesis Of Oxyalkylated Derivatives (3-9)supporting

confidence: 86%

“…Using methodology designed by our research group [17] with some modifications in temperature and reaction time, we obtained five new oxyalkylhydrochalcones 4-6, 8 and 9 and two known dihydrochalcones identified as 2'-Hydroxy-4'-methoxydihydrochalcone (3) [22] and dihydrocordoin (7) [23].…”

Section: Chemistrymentioning

confidence: 99%

Synthesis and Anti-Saprolegnia Activity of New 2’,4’-Dihydroxydihydrochalcone Derivatives

et al. 2020

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In the present study, seven 2’,4’-dihydroxydihydrochalcone derivatives (compounds 3–9) were synthesized and their capacity as anti-Saprolegnia agents were evaluated against Saprolegnia parasitica, S. australis, S. diclina. Derivative 9 showed the best activity against the different strains, with minimum inhibitory concentration (MIC) and minimum oomyceticidal concentration (MOC) values between 100–175 μg/mL and 100–200 μg/mL, respectively, compared with bronopol and fluconazole as positive controls. In addition, compound 9 caused damage and disintegration cell membrane of all Saprolegnia strains over the action of commercial controls.

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“…The results of the experiment showed that the highest activity against M. tuberculosis was noticed for extracts of C. arbuscula , E. nigrum , and J. communis roots, Calluna vulgaris aerial parts, and Myrica gale roots and stems (93% to 99% inhibition at 100 μg/mL). The fruit of black crowberry also contains 2′-methoxy-4′-hydroxy-α- and β–dihydrochalcone with antimicrobial activity [51]. Anti-mycobacterial activity of the plant extract was given by the presence of two chalcone derivatives that exhibited selective anti-mycobacterial activity (IC 50 values of 23.8 and 32.8 μM, respectively) in comparison to mammalian (HEK 293) cells (IC 50 values of 109 and 249 μM, respectively), which was confirmed by a study by Li et al [52].…”

Section: Health Promoting Activity Of Black Crowberry Fruitmentioning

confidence: 99%

Black Crowberry (Empetrum nigrum L.) Flavonoids and Their Health Promoting Activity

et al. 2016

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Nowadays, much research attention is focused on underutilized berry crops due to the high antioxidant activity of fruits. Black crowberry (Empetrum nigrum L.) represents an important source of flavonols (quercetin, rutin, myricetin, naringenin, naringin, morin, and kaempferol) and anthocyanins. The fruit components could be utilised as natural colourants or as a part of functional foods and, because of the high antioxidant activity, the berries of black crowberry can be used in the treatment of diseases accompanied with inflammation, or as an effective antibacterial and antifungal remedy. Moreover, the reduction of lipid accumulation and total cholesterol as well as an improvement of postprandial hyperglycaemia have been proven. This review summarizes for the first time the main antioxidants (flavonoids) of black crowberry fruits, with a focus on their health promoting activity.

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Cited by 15 publications

References 7 publications

Efficient separation of five flavonoids from Oxytropis falcata bunge by high-speed counter-current chromatography and their anticancer activity

Efficient separation of five flavonoids from Oxytropis falcata bunge by high-speed counter-current chromatography and their anticancer activity

Synthesis and Anti-Saprolegnia Activity of New 2’,4’-Dihydroxydihydrochalcone Derivatives

Black Crowberry (Empetrum nigrum L.) Flavonoids and Their Health Promoting Activity

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