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Faccenda, Filippo; Lunelli, F.; Gandolfi, A.; Bozzi, Rıccardo

doi:10.4194/1303-2712-v18_7_06

Cited by 2 publications

(1 citation statement)

References 16 publications

(23 reference statements)

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“…SSR-markers were proven to be essential in studies of the genetic structure of populations for providing management of fish stocks (Olagunju, 2019). Analysis of literature sources in recent years shows that microsatellites were predominantly used in genetic studies of rainbow trout in different countries (Barat et al, 2015;Abadía-Cardoso et al, 2016;Faccenda et al, 2018) to assess the genetic variability of stocks and evaluation of their relationships, reconstruction of the admixture history. Abadía-Cardoso et al (2016) showed the possibility of identifying the differ-ences in natural populations of Oncorhynchus mykiss Walbaum, 1792 from individuals of farmed stocks and assessing the impact on rainbow trout from natural water sources during stocking.…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of rainbow trout Oncorhynchus mykiss (Salmoniformes, Salmonidae) from aquaculture by DNA-markers

et al. 2021

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The rational use of valuable fish species from aquaculture is difficult to implement without knowledge of the state of the genetic structure of local stocks. Different types of DNA markers can be used to achieve the goals of selection and breeding work. The genetic structure of a local stock of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) (Salmoniformes, Salmonidae) farmed in Ukraine was studied using DNA-markers: microsatellite (SSR-markers – simple-sequence repeats-markers) and intermicrosatellite (ISSR – inter-simple sequence repeat). Five fragments of trinucleotide microsatellite motifs with a single anchor nucleotide at the 3'-end were used as a primer for analysis by the ISSR-PCR method. Totally, 85 amplicons were obtained across the five loci, of which 92.9% were polymorphic. The total number of alleles ranged from 10 (marker (ACC)₆G) to 23 (marker (AGC)₆G). The following monomorphic amplicons were determined for the studied local stock of rainbow trout: according to marker (CTC)₆C – 770 and 520 bp bands, for the marker (GAG)₆C – 345, 295 and 260 bp, and for the marker (AGC)₆C – 350 bp. The average number of polymorphic bands per locus was 15.8. The selected ISSR primers had a level of polymorphic information content above the average. The most effective markers for molecular-genetic analysis of rainbow trout were (AGC)₆G and (AGC)₆C according to the percentage of polymorphic bands, marker index, effective multiplex ratio and resolving power. The selected ISSR loci allow the genetic structure of the studied local stock to be characterized using the total and the effective number of alleles per locus (Na and Ne were 1.9 and 1.4, respectively), the Shannon index (average value I was 0.4) and the unbiased expected heterozygosity (mean uHe = 0.3). Microsatellite-based analysis showed features of the genetic structure of the local stock of rainbow trout at six microsatellite loci (OMM 1032, OMM 1077, OMM 1088, Str 15, Str 60, Str 73). Allelic diversity was established and alleles with the highest frequency and most typical for the given stock were identified. The Shannon index and unbiased expected heterozygosity were determined using SSR-markers and were 1.42 and 0.79, respectively. This depicts the complexity of the population structure, a high level of genetic diversity and indicates a high level of heterozygosity of local stock. The “gene pool profile” established as a result of ISSR-PCR in the future will help to differentiate local stocks of rainbow trout in aquaculture of Ukraine. Microsatellite markers provide the ability to determine individual features of genetic variation of local populations and to conduct the management of genetic resources on fish farms.

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Section: Introductionmentioning

confidence: 99%

Genetic structure of rainbow trout Oncorhynchus mykiss (Salmoniformes, Salmonidae) from aquaculture by DNA-markers

et al. 2021

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Effect of a Guar Meal Protein Concentrate in Replacement of Conventional Feedstuffs on Productive Performances and Gut Health of Rainbow Trout (Oncorhynchus mykiss)

Roncarati,

Galosi,

Di Cerbo

et al. 2024

Fishes

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A trial was performed to investigate the effect of a proprietary guar protein concentrate, as a partial replacement of conventional protein sources, on the productive performances of rainbow trout through the growing phase. A total of 2700 rainbow trout were reared in 3 m3, 12 concrete tanks for 90 days. Three diets were formulated to contain a protein level of 43% and a lipid content of 25.3% by replacing 0% (CD), 5% (D5), and 15% (D15) of conventional protein sources with guar protein concentrate. The final mean weight was similar between D5 and CD, significantly higher than D15. The same trend was observed in weight gain and specific growth rate. The feed conversion rate had the most favourable performances in D5 and CD. Feed palatability was higher in CD and D5 than in D15. Histological intestinal score showed significant differences (p < 0.05) between the groups with the highest values in CD and the lowest in D15. Significant differences were observed for goblet cell hyperplasia with higher values in the CD group. Based on this trial, the 5% guar protein concentrate inclusion gave the best zootechnical results.

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Cited by 2 publications

References 16 publications

Genetic structure of rainbow trout Oncorhynchus mykiss (Salmoniformes, Salmonidae) from aquaculture by DNA-markers

Genetic structure of rainbow trout Oncorhynchus mykiss (Salmoniformes, Salmonidae) from aquaculture by DNA-markers

Effect of a Guar Meal Protein Concentrate in Replacement of Conventional Feedstuffs on Productive Performances and Gut Health of Rainbow Trout (Oncorhynchus mykiss)

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