Untitled

Abstract

In human milk we previously found catalytic antibodies (abzymes) catalyzing hydrolysis of DNA, RNA, NMP, NDP, and NTP and also phosphorylation of proteins and lipids. In the present study we have analyzed nuclease activities of antibodies in blood of women during pregnancy and lactation. Blood of healthy male and female volunteers lacked catalytically active antibodies, whereas antibodies from blood of pregnant women hydrolyzed DNA and RNA and their relative activity varied over a wide range. Relative blood abzyme activities significantly increased after delivery and at the beginning of lactation. The highest abzyme activity was observed in blood of parturient women. Although the dynamics of changes in antibody DNase activity during pregnancy was rather individual for each woman, there was a common trend in the increase in antibody activity in the first and/or third trimester of the pregnancy. The DNase activity of IgG and IgM from blood of healthy pregnant women was 4-5 times less than that from pregnant women with pronounced autoimmune thyroiditis.

Natural Catalytic Antibodies – Abzymes

“…Taken together, in contrast to IgG from normal women before pregnancy and IgG from men, IgG from the serum of healthy pregnant women has DNAse and RNase activities which increase remarkably after the beginning of lactation [140], and this increase is much more significant in mothers who suffered from viral direases, for example, influenza or allergic diseases [140], or in females with induced AD during pregnancy [69]. Our data and the data in the literature show that the immune system of pregnant women may be stimulated by proteins and NAs in a different manner to that of normal humans.…”

“…Therefore, we have compared the levels of DNase activity of IgG and IgM from the blood of pregnant healthy females without pathologies and women with an acute form of HT. It was shown that DNAse activity of Abzs in women with AD induced by pregnancy is overall about 4-5 times higher than this activity in the case of healthy pregnant women [140].…”

“…The maximal activity of one of the samples used was taken as 100%. The details of the analysis are given in [140]. As mentioned above, we have shown that the blood of patients with HT contains DNase and RNase Abzs [53,56,141,142].…”

“…However, there was no data about the presence of Abzs in the blood of women during their pregnancy and in early post-natal period. Taking this into account, we have recently studied the dynamics of RNase and DNAse activities of Abs from the blood of pregnant women [140]. It was shown that the relative catalytic activity of Abs in the hydrolysis of DNA and RNA from the blood of different pregnant women could vary over a large range.…”

“…Different forms of plasmid DNA are marked as: C, circular; L, linear; F and ODN, fragments of DNA of different length including ODNs. The details of the analysis are given in [140].…”