Untitled

Abstract

The influence of MX(3-Chloro-4(Dichloromethyl)-5-Hydroxy-2(5H)- Furanone), a stronglymutagenic compound, on the temperature dependence of the dcelectrical conductivity of collagen as a function of time was studied.Collagen was immersed in MX solution, next dried and pressed intotablets. The MX concentration was measured by HPLC analysis.The reduction of MX concentration to 10% of the initial value wasobserved in the presence of collagen in the solution, whereas in thecontrol solution concentration of MX decreased to 70% of the initialvalue. Measurements of electrical conductivity were performed for thetemperature range 295-453K and activation energies for the chargeconduction process were calculated. Within the temperature range295-340K, the presence of MX decreased electrical conductivity ofcollagen. Calculated activation energies were typical for dry proteins.Within the temperature range 295-320K activation energy decreasedwith time, probably due to the stronger interactions in thecollagen-water-MX system. For temperatures between 320-410 and430-450K the activation energy was not time dependent and theapplication of MX did not change the structure of the collagenmacromolecule. The temporary changes occurring at the lowertemperatures being due solely to changes in the collagen-waterinteractions.

Electrical Conductivity of Fish Skin Collagen in the Temperature Range 290-380 K

“…The disappearance of the peak was probably due to the evacuation of free water and bound water, which prior the diffusion out of collagen, was transformed into free water. Similar results were presented in other papers [10,11]. Also according to thermogravimetrical studies on dry collagen, the release of bound water take place in the temperature range 313-443 K, with the highest rate at 353-373 K [12].…”

Temperature dependence of electric conductivity of bamboo coral skeleton and glass sponge spicules, the marine origin biomaterials

“…Such a difference in the denaturation temperature is not surprising. Denaturation temperature of dry collagen depends on its hydration, annealing processes, previous collagen treatment and presence of other substances mainly mineral components such as apatite [4,7,[10][11][12]. According to divers data, the denaturation temperature of collagen varies from 160°C even to 240°C [11][12][13][14].…”

“…Electric conductivity is sensitive to any change in chemical composition and structure of studied material as a result of which along with its temperature dependence of electric conductivity enable the assessment of modification of the basic material. It also permits to state whether the observed changes are permanent or temporary [4].…”

DC Electrical Conductivity in Studies on Solid-State Proteins

“…However, the reduction in electric conductivity was noticed, it was also shown that MX had no effect on the structure of the collagen molecule itself. Moreover, the fall in activation energy of charge conduction process with time to values typical for non-treated collagen, suggested, that the influence of MX was a temporary effect [18,36].…”

“…Electric conductivity is sensitive to any change in chemical composition, structure of studied material and also permits to state whether the observed changes are permanent or temporary [18]. Measurements of σ-T are applied in studies on polymers and complexes as well [19].…”