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Szarka, B.; Dévényi, M.; Mórocz, S.

doi:10.1023/a:1012699332546

Cited by 7 publications

(1 citation statement)

References 13 publications

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“…Adding foreign species-derived ovaries to isolated microspore cultures was also reported to enhance androgenesis in several studies. Microspore-derived wheat embryos were produced through the use of barley ovaries as nurse agents (Bruins et al 1996); while, co-culture of barley ovaries increased viability of maize microspores (Szarka et al 2001). Zheng et al (2003) observed that wheat ovaries significantly improved the efficiency of maize microspore cultures, and Coronado et al (2005) reported that wheat ovary co-cultures increased efficiency of androgenesis in recalcitrant barley genotypes.…”

Section: Discussionmentioning

confidence: 99%

Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat

Lantos

Juhász²,

Somogyi³

et al. 2009

Plant Cell Tiss Organ Cult

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The influence of the developmental stage of microspores on establishing isolated microspore cultures of three Hungarian ('Szegedi 80', 'Szegedi 178', and 'Remény') and three Spanish ('Jeromin', 'Jariza', and 'Jaranda') pepper genotypes was investigated. Donor anthers containing 80% uninucleated and 20% binucleated microspores yielded the highest frequency of successful microspore cultures. Cocultures with wheat, line 'CY-45', ovaries exhibited enhanced frequency of embryoid production than those with pepper ovaries. Differences in efficiency of isolated pepper microspore culture establishment were observed among different pepper genotypes. Green plantlets were regenerated from microspore-derived embryoids, but some were exhibited abnormal growth habits, such as leaf rosetting. A total of seven fertile microspore-derived plants were obtained, including three 'Jariza', three 'Jaranda', and a single 'Szegedi 80' plant.

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Section: Discussionmentioning

confidence: 99%

Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat

Lantos

Juhász²,

Somogyi³

et al. 2009

Plant Cell Tiss Organ Cult

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Using New Methodsin Conventional Breeding of Maize (Zea Mays L.)

Pepó

2004

CEREAL RESEARCH COMMUNICATIONS

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Gametic embryos of maize as a target for biolistic transformation: comparison to immature zygotic embryos

et al. 2002

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The aim of the present study was to determine the suitability of maize gametic embryos of three ETH genotypes as a target for biolistic transformation. We studied parameters considered essential for a successful transformation, such as the frequency of secondary embryo formation, their regeneration ability and the transient transgene expression. Transformable zygotic embryos of one of the ETH genotypes were used as positive control. Our results indicate that gametic embryos can potentially be transformed by particle bombardment, since they responded positively to all the studied parameters, although with lower efficiencies than the zygotic embryos. In particular, differences were found in the rate of secondary embryogenesis and the density of transformed cells.

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Untitled

Cited by 7 publications

References 13 publications

Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat

Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat

Using New Methodsin Conventional Breeding of Maize (Zea Mays L.)

Gametic embryos of maize as a target for biolistic transformation: comparison to immature zygotic embryos

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