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Abstract

Novel homotetranuclear Cu(II) and heteronuclear Cu(II)-Mn(II) complexes with tetrathioether-tetrathiol moiety have been prepared and their DNA relaxation activities with plasmid pCYTEXP (5kb) were electrophoretically established. The cleavage products analyzed by neutral agarose gel electrophoresis indicated that the interaction of the metal complexes with supercoiled plasmid DNA yielded linear, nicked or degraded DNA. The relaxation activities of both homo- and heterotetranuclear (SK4) complexes are time- and concentration-dependent. The findings suggest that SK4 with potent nucleolytic activity is a good nuclease substitute in the presence ofcooxidant. Furthermore, the observation of induction of DNA into smaller fragments by SK4 is also significant.

DNA hydrolysis promoted by di- and multi-nuclear metal complexes

“…Dulger group has shown double-stranded DNA cleavage mediated by heterotetranuclear Cu 2+ Mn 2+ complexes based on a tetrathioether-tetrathiol ligand [91]. In the absence of external oxidants, the tetranuclear complex cleaved supercoiled DNA to yield linear DNA within 5 min at 10-50 M concentrations.…”

Catalytic hydrolysis of DNA by metal ions and complexes

“…The mechanisms of cleavage were not determined and are presumed to be hydrolytic. Dulger et al [52] have shown ds DNA cleavage by heterotetranuclear Cu 2+ /Mn 2+ complexes based on a tetrathioether-tetrathiol ligand. In the absence of external oxidants, the tetranuclear complex cleaved supercoiled DNA to yield linear DNA within 5 min at 10±50 M concentrations.…”

Synthesis, characterization, DNA interaction, molecular docking, and α-amylase and α-glucosidase inhibition studies of a water soluble Zn(ii) phthalocyanine

“…The pBR322 plasmid DNA cleavage activity of ZnPc-2 was also studied by agarose gel electrophoresis. 59 The supercoiled pBR322 plasmid DNA was incubated with varying concentrations of ZnPc-2 . The restriction endonuclease Eco RI was used to identify the linear form of pBR322 plasmid DNA.…”