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Bavage, Adrian D.; Davies, I.; Robbins, Mark P.; Morris, Phillip

doi:10.1023/a:1005841214470

Cited by 51 publications

(7 citation statements)

References 19 publications

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“…To date, the biosynthetic pathways for CTs and a large number of polyphenolic compounds have been well established [7], [22]. DFR encodes a key enzyme in the later steps of CT synthesis in plants, and overexpression of a DFR gene in the forage legume Lotus corniculatus resulted in an alteration of CT levels [48], [49]. To further determine the function of PtrDFR s in the CT biosynthetic pathway, their ORFs, flanked by the 35S promoter, were introduced into P. tomentosa Carr.…”

Section: Resultsmentioning

confidence: 99%

Molecular Cloning and Characterization of Two Genes Encoding Dihydroflavonol-4-Reductase from Populus trichocarpa

et al. 2012

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Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a rate-limited enzyme in the biosynthesis of anthocyanins and condensed tannins (proanthocyanidins) that catalyzes the reduction of dihydroflavonols to leucoanthocyanins. In this study, two full-length transcripts encoding for PtrDFR1 and PtrDFR2 were isolated from Populus trichocarpa. Sequence alignment of the two PtrDFRs with other known DFRs reveals the homology of these genes. The expression profile of PtrDFRs was investigated in various tissues of P. trichocarpa. To determine their functions, two PtrDFRs were overexpressed in tobacco (Nicotiana tabacum) via Agrobacterium-mediated transformation. The associated color change in the flowers was observed in all 35S:PtrDFR1 lines, but not in 35S:PtrDFR2 lines. Compared to the wild-type control, a significantly higher accumulation of anthocyanins was detected in transgenic plants harboring the PtrDFR1. Furthermore, overexpressing PtrDFR1 in Chinese white poplar (P. tomentosa Carr.) resulted in a higher accumulation of both anthocyanins and condensed tannins, whereas constitutively expressing PtrDFR2 only improved condensed tannin accumulation, indicating the potential regulation of condensed tannins by PtrDFR2 in the biosynthetic pathway in poplars.

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Section: Resultsmentioning

confidence: 99%

Molecular Cloning and Characterization of Two Genes Encoding Dihydroflavonol-4-Reductase from Populus trichocarpa

et al. 2012

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“…Particularly, anthocyanins are one of the richest plant natural pigments with significant economic values in the horticulture industry [ 6 , 10 , 11 , 12 , 13 ]. Since the first success of Petunia flower color engineering was achieved using the first DFR cDNA cloned from maize [ 13 ], multiple homologs have been cloned to engineer anthocyanins [ 1 , 14 , 15 ] and proanthocyanidins [ 16 , 17 ]. DFR also plays a key role in transcription factor-based anthocyanin engineering.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Functional Characterization of a Dihydroflavonol 4-Reductase from Vitis bellula

Zhu

Qin

et al. 2018

Molecules

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Vitis bellula is a new grape crop in southern China. Berries of this species are rich in antioxidative anthocyanins and proanthocyanidins. This study reports cloning and functional characterization of a cDNA encoding a V. bellula dihydroflavonol reductase (VbDFR) involved in the biosynthesis of anthocyanins and proanthocyanidins. A cDNA including 1014 bp was cloned from young leaves and its open reading frame (ORF) was deduced encoding 337 amino acids, highly similar to V. vinifera DFR (VvDFR). Green florescence protein fusion and confocal microscopy analysis determined the cytosolic localization of VbDFR in plant cells. A soluble recombinant VbDFR was induced and purified from E. coli for enzyme assay. In the presence of NADPH, the recombinant enzyme catalyzed dihydrokaempferol (DHK) and dihydroquercetin (DHQ) to their corresponding leucoanthocyanidins. The VbDFR cDNA was introduced into tobacco plants via Agrobacterium-mediated transformation. The overexpression of VbDFR increased anthocyanin production in flowers. Anthocyanin hydrolysis and chromatographic analysis revealed that transgenic flowers produced pelargonidin and delphinidin, which were not detected in control flowers. These data demonstrated that the overexpression of VbDFR produced new tobacco anthocyanidins. In summary, all data demonstrate that VbDFR is a useful gene to provide three types of substrates for metabolic engineering of anthocyanins and proanthocyanidins in grape crops and other crops.

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“…For example, expression of a cDNA encoding Antirrhinum majus L. dihydroflavonol reductase in hairy roots of Lotus corniculatus L. caused an increase in the content, and an alternation in the structure, of condensed tannins in a manner consistent with the substrate specificity of the trangene product (Bavage et al 1997). Other interesting example involves the introduction of a heterologous tryptophan decarboxylase gene into hairy root cultures of Peganum harmala L., which accumulate two biosynthetically related, tryptamine-derived secondary metabolites: serotonin and b-carboline alkaloids (Berlin et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Influence of Agrobacterium rhizogenes on induction of hairy roots and benzylisoquinoline alkaloids production in Persian poppy (Papaver bracteatum Lindl.): preliminary report

Rostampour

Sohi

Jourabchi

et al. 2009

World J Microbiol Biotechnol

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Persian poppy (Papaver bracteatum Lindl.) is an important medicinal plant and source of the opium alkaloids codeine, morphine and thebaine. Transgenic root cultures of P. bracteatum Lindl. are well-defined model systems to investigate the molecular and metabolic regulation of benzylisoquinoline alkaloid biosynthesis. Agrobacterium rhizogenes was able to produce hairy roots on wounded Persian poppy seedlings. Excised shoots from 7-day-old Persian poppy were co-cultivated with the A. rhizogenes strain R15834 carrying the pBI121 binary vector. All media, except for the co-cultivation medium, included 40 mg l -1 paromomycin to select for pBI121 transformants and 200 mg l -1 cefotaxime to eliminate the Agrobacterium. Eight weeks after infection, paromomycinresistant roots appeared on 45-50% of explants maintained on hormone-free medium. Isolated hairy roots were propagated in liquid medium containing 1.0 mg l -1 1-naphthaleneacetic acid to promote rapid growth. Also, callus induction and shoot regeneration of transformed Calli in vitro was achieved on B5 medium containing 1.0 mg l -1 1-naphthaleneacetic acid. Detection of the neomycin phosphotransferase gene and GUS histochemical localization confirmed the integrative transformation of root cultures. This is the first study to illustrate useful protocol to introduce foreign genes into transgenic Persian poppy hairy root cultures using A. rhizogenes strain R15834.

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Cited by 51 publications

References 19 publications

Molecular Cloning and Characterization of Two Genes Encoding Dihydroflavonol-4-Reductase from Populus trichocarpa

Molecular Cloning and Characterization of Two Genes Encoding Dihydroflavonol-4-Reductase from Populus trichocarpa

Molecular Cloning and Functional Characterization of a Dihydroflavonol 4-Reductase from Vitis bellula

Influence of Agrobacterium rhizogenes on induction of hairy roots and benzylisoquinoline alkaloids production in Persian poppy (Papaver bracteatum Lindl.): preliminary report

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