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Nieves, Nadina; Lorenzo, José Carlos; Blanco, M.; González, José Ignacio López; Peralta, Huaman; Hernández, Martha; Santos, Ramón; Concepción, Oscar; Borroto, C.G; Borroto, E.; Tapia, R.; Martínez, M. E.; Fundora, Z.; González, Augusto

doi:10.1023/a:1006101714352

Cited by 29 publications

(6 citation statements)

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“…Also, encapsulation of propagules that were produced in vitro could reduce the cost of micropropagation of plantlets for commercialization and final delivery (Chu 1995, Nieves et al 2003. This technology may be of value in breeding programs and allows the propagation of many elite genotypes in a short time (Nieves et al 1998). In some of the horticultural crops, seeds propagation is not successful due to: a. heterozygosity of seeds particularly in cross pollinated crops, b. minute seed size (orchids), c. presence of reduced endosperm, d. some seeds require mycorrhizal fungi association for germination (orchids), e. no seeds are formed.…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

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Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion:The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations

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Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

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“…Synthetic seeds can be also called artificial seeds or synseeds. Somatic embryogenesis is considered the most appropriate technique for rapid-large scale micropropagation and synseed technology [14][15][16], but somatic embryogenesis technique is not well established in many plant species. In such cases, the encapsulation of shoot tips and/or axillary buds provides an alternative plant material to produce synseeds [13,17].…”

Section: Introductionmentioning

confidence: 99%

“…Synseed consists of somatic embryo or alternative plant materials encapsulated within a synthetic endosperm which containing nutrient reserves and other additives [15,[19][20][21]. These structures could also be encapsulated further with a synseed coats to suit mechanical handling and planting [22].…”

Section: Introductionmentioning

confidence: 99%

Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat

Hassanein¹,

Ibraheim²,

Galal³

et al. 2011

AJPS

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“…La elaboración de semillas sintéticas ha permitido la propagación y preservación a largo plazo de embriones somáticos de orquídeas (Teixeira da Silva et al, 2014) y agaves (Chandrasekhara et al, 2012). Por otro lado, el encapsulado de tejidos como segmentos nodales de plantas con importancia frutícola (Rai et al, 2009) se ha aplicado en cítricos (Nieves et al, 1998) en plantas medicinales como Centella asiatica (Prasad et al, 2014), y en alfalfa (Medicago sativa L.) (Fujii et al, 1992). En caña de azúcar (Saccharum officinarum L.) variedad CP-5243, los embriones encapsulados se han plantado directamente en campo (Nieves et al, 2003).…”

Section: Introductionunclassified

Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae)

et al. 2019

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Antecedentes y Objetivos: Castilleja tenuiflora es una de las plantas mexicanas, silvestres y medicinales cultivadas in vitro que presentan actividad biológica antiinflamatoria y antiulcerogénica. La encapsulación de microestacas es una opción para propagar y prolongar el almacenamiento de esta planta. El objetivo de este estudio fue establecer un método para la encapsulación y conservación de microestacas de C. tenuiflora sin afectar el contenido de sus compuestos.Métodos: Las cápsulas fueron elaboradas con alginato de sodio en complejo con cloruro de calcio dihidratado como matriz y microestacas de tejido proveniente de plántulas cultivadas in vitro. Se probaron diferentes concentraciones y tiempos de polimerización, así como diferentes tiempos de almacenamiento y temperatura, para posteriormente analizar los metabolitos mayoritarios de los extractos metanólicos mediante cromatografía (HPLC) de las plantas resultantes.Resultados clave: La combinación para la producción de cápsulas con microestacas es alginato de sodio al 3% y cloruro de calcio dihidratado al 50 mM, con 25 minutos de polimerización. Las plántulas derivadas de semillas sintéticas, almacenadas por 100 días presentaron los mismos compuestos que las plantas donantes.Conclusiones: La encapsulación de microestacas de C. tenuiflora con alginato de sodio y cloruro de calcio dihidratado permite el almacenamiento del material vegetal y se mantienen sus compuestos principales, por lo que es una alternativa para mantener el genotipo de plantas elite y contrarrestar el saqueo de plantas silvestres.

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Cited by 29 publications

References 10 publications

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat

Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae)

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