99mTc-sestamibi to monitor treatment with antisense oligodeoxynucleotide complementary to MRP mRNA in human breast cancer cells

Kinuya, Seigo; Bai, Jingming; Shiba, Kazuhiro; Yokoyama, Keitaro; Mori, Hirofumi; Fukuoka, Masayoshi; Watanabe, Naoto; Shuke, Noriyuki; Michigishi, Takatoshi; Tonami, Norihisa

doi:10.1007/bf02985587

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…Technetium-99 m hexakis-2-methoxyisobutylisonitrile, Tc-99 m-sestamibi ( 99m Tc-MIBI) is a cationic lipophilic radiopharmaceutical commonly used in nuclear cardiology that has been reported to be used in several tumour types including those of the breast, lung, thyroid, brain, head and neck, gastrointestinal tract, solid tumours of bones and soft tissues and lymphomas [13-16]. The human breast adenocarcinoma MCF-7 cell line was used because it is a commonly available human breast cancer in vitro model.…”

Section: Introductionmentioning

confidence: 99%

Study of the cytotoxicity of asiaticoside on rats and tumour cells

Al-Saeedi

2014

BMC Cancer

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BackgroundCancer chemoprevention is considered one of the most promising areas in current cancer research, and asiaticoside, which is derived from the plant Centella asiatica, has a relative lack of systemic toxicity. The purpose of this study was to investigate whether asiaticoside is effective against 7,12-dimethylbenz(a)anthracene (DMBA)-induced carcinogenicity in vitro (MCF-7 and other cells) and in vivo (DMBA-induced rat cancer).MethodsAn MTT assay was performed involving the treatment of MCF-7 cells for 48 h with H2O2 alone and H2O2 + different asiaticoside concentrations. Flow cytometry was performed, and the level of caspase 3, tumour necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1) were quantified. Adult female Sprague–Dawley (SD) rats were divided into five groups designated I (control), II (DMBA-induced cancer), III (pre- and post-treatment with asiaticoside (200 μg/animal) in DMBA-induced cancer), IV (post-treatment with asiaticoside in DMBA-induced cancer), and V (treated with asiaticoside alone, drug control). Twelve weeks post-DMBA, rats developed mammary tumours. Rats either were sacrificed or imaged with MIBI. Histological examination of tumour tissues was performed. Tumour MIBI uptake ratios were determined. The data are expressed as the means ± standard deviation. Appropriate t-test and ANOVA statistical methods were used to compare data.ResultsThe IC50 of asiaticoside for MCF-7 cells was determined to be 40 μM. Asiaticoside has potential for hydrogen peroxide cytotoxicity, and the caspase-3 activity increased with increasing asiaticoside dose in MCF-7 cells treated for 48 h. The expression of the cytokines TNF-α and IL-1β was significantly decreased and correlated with MIBI uptake ratios in vitro and in vivo after asiaticoside administration.ConclusionThis study demonstrates that asiaticoside is effective in vitro and in vivo in inducing apoptosis and enhancing anti-tumour activity.

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Section: Introductionmentioning

confidence: 99%

Study of the cytotoxicity of asiaticoside on rats and tumour cells

Al-Saeedi

2014

BMC Cancer

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“…Thecat ionic lipophilic agent 99m Tc-hexakis-2-methoxyisobutylisonitrile, or 99m Tc-sestamibi ( 99m Tc-MIBI), is widely used for myocardial perfusion imaging (1). 99m Tc-MIBI has been reported to accumulate within the mitochondria and cytoplasm of cells on the basis of transmembrane electrical potentials (2,3). 99m Tc-tetrofosmin ( 99m Tc-Tfos) is a monovalent cationic lipophilic agent (diphosphine group) (4,5) that was originally developed for myocardial perfusion imaging.…”

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confidence: 99%

Effect of Asiaticoside on 99mTc-Tetrofosmin and 99mTc-Sestamibi Uptake in MCF-7 Cells

Al-Saeedi

Bitar²,

Pariyani³

2011

Journal of Nuclear Medicine Technology

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“…Tracer's uptake was increased practically linearly with incubation times up to 30 min. Previous studies on 99m Tc-MIBI have used an incubation period of 30 min [ 13 , 14 ]. Therefore, an incubation time of 30 min was chosen in all experiments ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

Comparison of ^99mTc-Tetrofosmin and ^99mTc-Sestamibi Uptake in Glioma Cell Lines: The Role of P-Glycoprotein Expression

Alexiou

Xourgia

Vartholomatos

et al. 2014

International Journal of Molecular Imaging

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99mTc-Tetrofosmin (99mTc-TF) and 99mTc-Sestamibi (99mTc-MIBI) are SPECT tracers that have been used for brain tumor imaging. Tumor's multidrug resistance phenotype, namely, P-glycoprotein (p-gp), and the multidrug resistance related proteins (MRPs) expression have been suggested to influence both tracers' uptake. In the present study we set out to compare 99mTc-TF and 99mTc-MIBI uptake in high-grade glioma cell lines and to investigate the influence of gliomas p-gp expression on both tracers' uptake. We used four glioma cell lines (U251MG, A172, U87MG, and T98G). The expression of p-gp protein was evaluated by flow cytometry. Twenty μCi (7.4·105 Bq) of 99mTc-TF and 99mTc-MIBI were used. The radioactivity in the cellular lysate was measured with a dose calibrator. P-gp was significantly expressed only in the U251MG cell line (P < 0.001). In all gliomas cell lines (U251MG, U87MG, A172, and T98G) the 99mTc-TF uptake was significantly higher than 99mTc-sestamibi. The U251MG cell line, in which significant p-gp expression was documented, exhibited the strongest uptake difference. 99mTc-TF uptake was higher than 99mTc-MIBI in all studied high-grade glioma cell lines. Thus, 99mTc-TF may be superior to 99mTc-MIBI for glioma imaging in vivo.

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99mTc-sestamibi to monitor treatment with antisense oligodeoxynucleotide complementary to MRP mRNA in human breast cancer cells

Abstract: Effects of AS-ODN administration on MRP function can be monitored via assessment of cellular uptake of 99mTc-MIBI.

Cited by 4 publications

References 19 publications

Study of the cytotoxicity of asiaticoside on rats and tumour cells

Study of the cytotoxicity of asiaticoside on rats and tumour cells

Effect of Asiaticoside on 99mTc-Tetrofosmin and 99mTc-Sestamibi Uptake in MCF-7 Cells

Comparison of ^99mTc-Tetrofosmin and ^99mTc-Sestamibi Uptake in Glioma Cell Lines: The Role of P-Glycoprotein Expression

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99mTc-sestamibi to monitor treatment with antisense oligodeoxynucleotide complementary to MRP mRNA in human breast cancer cells

Abstract: Effects of AS-ODN administration on MRP function can be monitored via assessment of cellular uptake of 99mTc-MIBI.

Cited by 4 publications

References 19 publications

Study of the cytotoxicity of asiaticoside on rats and tumour cells

Study of the cytotoxicity of asiaticoside on rats and tumour cells

Effect of Asiaticoside on 99mTc-Tetrofosmin and 99mTc-Sestamibi Uptake in MCF-7 Cells

Comparison of 99mTc-Tetrofosmin and 99mTc-Sestamibi Uptake in Glioma Cell Lines: The Role of P-Glycoprotein Expression

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Comparison of ^99mTc-Tetrofosmin and ^99mTc-Sestamibi Uptake in Glioma Cell Lines: The Role of P-Glycoprotein Expression