99mTc-N4amG: Synthesis biodistribution and imaging in breast tumor-bearing rodents

Abstract

99mTc-N4-guanine (99mTc-N4amG) was synthesized and evaluated in this study. Cellular uptake and cellular fraction studies were performed to evaluate the cell penetrating ability. Biodistribution and planar imaging were conducted in breast tumor-bearing rats. Up to 17%ID uptake was observed in cellular uptake study with 40% of 99mTc-N4amG was accumulated in the nucleus. Biodistribution and scintigraphic imaging studies showed increased tumor/muscle count density ratios as a function of time. Our results demonstrate the feasibility of using 99mTc-N4amG in tumor specific imaging.

Looking for New [1,2,4,5]Tetrazines to Produce 99mTc‐Labelled Derivatives, with a Suitable Lipophilicity Balance for Use in Bioorthogonal Reactions

“…As a first approach to obtain new TZ with suitable lipophilicity balance, we explored the 99m Tc‐coordination‐moiety derived from 1,4,8,11‐tetrazacyclotetradecane (known as cyclam) which could yield cationic Tc(V) complexes. Consequently, from the common starting material (4) [23] and commercial TZs, two new TZs were prepared in moderate global yields ( (5) and (6) , Scheme 2). With these TZs, one resembling to parent compound (1) and the other to compound (2) , we expected that they would have different hydrophilic behaviour.…”

“…Compound (4) and bevacizumab-TCO were prepared as previously were reported. , [21,23] The used solvents were distilled prior to their use. If necessary, they were dried using established procedures.…”

Synthesis, quality control, and bio‐evaluation of 99mTc‐cyclophosphamide

“…It may be difficult to detect, but for several types of cancer, earlier it is detected, the better are the chances of treating it effectively. [20] Cyclophosphamide is alkylating agent which is most widely used of all antineoplastic drugs. In vivo functional imaging technique can help to diagnose diseases, drug planning, and predicting therapeutics response, which may be beneficial to patient and oncologist.…”