99mTc-EC-Guanine: Synthesis, Biodistribution, and Tumor Imaging in Animals

Abstract

Our results indicate that (99m)Tc-EC-Guan may be useful as a tumor proliferation imaging agent.

Molecular imaging of Bcr-Abl phosphokinase in a xenograft model

“…Ethylenedicysteine (EC) is the most recent and successful example of N 2 S 2 chelates (18–21). EC can be labeled with metallic isotopes easily and efficiently with high radiochemical purity and stability (12–14).…”

PET and SPECT Imaging of Tumor Proliferation

“…9). 158 Cell culture assays indicated that EC-Guan was incorporated in DNA, and there was no significant uptake difference between HSVTK overexpressed and normal groups. Biodistribution and scintigraphic imaging studies of 99m Tc-EC-Guanine showed increased tumor/tissue count density ratios as a function of time.…”

Thymidine kinase enzyme selective imaging radiopharmaceutical: 99mTc(CO)3-Ganciclovir

“…Thus, researchers reported that future quantitative studies will be needed to understand using these radiolabeled agents for imaging HSV1-tk reporter gene expression with PET [15]. Yang et al [16] synthesized 99m Tcethylenedicysteine-guanine ( 99m Tc-EC-Guan) and evaluated its cell proliferation selecting A-549, ovarian OCA-3, PC-3 and HSV-tk gene transfected PC-3 cancer cell lines. They reported that there was no significant uptake difference between HSV-TK over expressed and normal groups.…”