99. Importance, involvement and detection of glassy state in plant meristematic tissue for cryopreservation

Zámečnı́k, Jiřı́; Bilavčík, Alois; Faltus, Miloš

doi:10.1016/j.cryobiol.2007.10.102

Cited by 3 publications

(5 citation statements)

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“…When taking advantage of the glassy state for preservation purposes, often the difficulty lies in the prediction and control of this temperature. Another problem is how to reach temperatures below T G , from room temperature, without ice being formed during the cooling process (the same problem arises when warming specimens to room temperature) [1,23]. The probability of ice formation increases with the time that the system is at a temperature comprised between T f (the equilibrium freezing temperature) and T G .…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Glass transition and heat capacity behaviors of plant vitrification solutions

et al. 2014

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Differential scanning calorimetry (DSC) was employed to investigate the vitrification and annealing behaviors of the most commonly used plant vitrification solutions (PVS). These solutions are employed to protect plant tissues towards ice formation and freeze injury, and help to the vitrification of these tissues, by globally reducing the intracellular fluids mobility. Glass transition temperatures (T g) and heat capacity increments (∆Cp) were determined for five solutions PVS1, PVS2, PVS2 mod, PVS3 and PVS3 mod, with different composition, and a range of cooling and warming rates was employed. Glass transitions showed clear and consistent temperature differences within vitrification solutions, which could be related to composition and water content. Roughly, two sets of T G values were obtained, those for PVS1 and 2, at-112 ºC and-114 ºC, respectively, and those for PSV3, at-90 ºC. The observed T g and ∆Cp, unexpectedly, did not significantly change within a wide range of cooling rates (from 5 ºC min-1 to liquid nitrogen quenching) and warming rates (from 5 to 20 ºC). Garlic shoot tips cryopreserved after the droplet method produced a similar result to that of the vitrification solutions employed. After quench cooling to temperatures below T g , repeated excursions to higher temperatures were made and the cooling and warming T g were recorded. These treatments had little or no effect over the PVS solutions T g , which remained practically constant. A direct practical consequence is that the plant vitrification solutions glass transition temperature does not significantly change with cryopreservation methods based on either direct plunging of samples into liquid nitrogen or employing closed cryovials.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Glass transition and heat capacity behaviors of plant vitrification solutions

et al. 2014

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“…Differential scanning calorimetry (DSC) is a well‐known technique used for studying thermal properties of thermally driven processes. It has been used for long in cryopreservation studies, as it yields interesting information on freezing and vitrification processes . Water freezing latent heat is large, so calorimetry is a sensitive method to monitor ice formation or thawing, even in small quantities.…”

Section: Introductionmentioning

confidence: 99%

“…Once vitrified, despite some time-depending phenomena still taking place, relatively large molecular reorganizations, as those required for ice formation, are not possible and specimens can be stored for hypothetically indefinite periods, without ice being formed. 5 A third step consists of the rewarming process, which must be performed quickly enough to avoid ice nucleation while crossing by the temperature region comprised between the glass transition temperature (T G ) and the equilibrium freezing point (T f ). [6][7][8] Progressive dehydration and rapid cooling have been found to avoid the formation and growth of lethal intracellular ice in plant cells.…”

Section: Introductionmentioning

confidence: 99%

“…It has been used for long in cryopreservation studies, as it yields interesting information on freezing and vitrification processes. 5,8,[25][26][27] Water freezing latent heat is large, so calorimetry is a sensitive method to monitor ice formation or thawing, even in small quantities. Besides the amount of ice formed, after knowledge of the system's water content, the ratio of frozen-unfrozen water may also be obtained.…”

Section: Introductionmentioning

confidence: 99%

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Glassy State and Cryopreservation of Mint Shoot Tips

Teixeira

González‐Benito

García

2013

Biotechnology Progress

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“…For comparison purposes, here we have defined two windows: from 0 to 2150 C and from 220 C to 2120 C. The first one, spanning from pure water equilibrium freezing point (the higher temperature where ice can exist, in any aqueous solution) to 2150 C, well below any expected glass transition in a living system, is largely overestimated over the real ice formation risk zone for systems in cryopreservation, as its T f will be always much lower than 0 C, due its high solute concentration and low water content. The variability in the glass transition temperature is high, being very sensitive to the content in water and other small size molecules, 28,29 but, for plant viable germplasm, is usually well over 2150 C. Moreover, the thermal gradient (DT, the difference between sample and surrounding media temperatures) is similar in the initial and final windows extremes, for both cooling and warming (using a 40 C bath). For example, for the 0 C to 2150 C window, DT would evolve from 196 C at the initial point, to 46 C at the window end-point, for cooling, and from an initial value of 190 C to a final one of 40 C, for warming.…”

Section: Discussionmentioning

confidence: 99%

Measurement of cooling and warming rates in vitrification‐based plant cryopreservation protocols

Teixeira

González‐Benito

García

2014

Biotechnology Progress

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Cryopreservation protocols include the use of additives and pretreatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (Tf ) and the glass transition (TG ) temperatures. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. Vitrification and droplet-vitrification techniques, frequently used cryopreservation protocols based in fast cooling, were studied, alongside with the corresponding warming procedures. A very fast data acquisition system, able to read very low temperatures, down to that of liquid nitrogen, was employed. Cooling rates, measured between -20°C and -120°C, ranged from ca. 5°C s(-1) to 400°C s(-1) , while warming rates spanned from ca. 2°C s(-1) to 280°C s(-1) , for the different protocols and conditions studied. A wider measuring window (0°C to -150°C) produced lower rates for all cases. The cooling and warming rates were also related to the survival observed after the different procedures. Those protocols with the faster rates yielded the highest survival percentages.

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99. Importance, involvement and detection of glassy state in plant meristematic tissue for cryopreservation

Cited by 3 publications

References 0 publications

Glass transition and heat capacity behaviors of plant vitrification solutions

Glass transition and heat capacity behaviors of plant vitrification solutions

Glassy State and Cryopreservation of Mint Shoot Tips

Measurement of cooling and warming rates in vitrification‐based plant cryopreservation protocols

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