968 IL-36γ is a strong inducer of IL-23A expression in human 3D psoriasis skin models consisting of lesional epidermal keratinocytes and dermal fibroblasts

Abstract

Skin microbiome has an important role as host guardian, contributing to several physiological functions including skin barrier maintenance and protection against pathogenic microorganisms. Skin microbiome varies according to geography, cultural and ethnic conditions and all these parameters interfere in skin-microorganism communication. In this study, we identified the skin microbiome of a South American group and studied its behavior when associated with a 3D skin model. Both metagenomic and transcriptomic evaluations were performed to understand the interaction between the microbiome and the skin in vitro. Altogether, our results suggest that although it is feasible to cultivate a wild type microbiome with a 3D skin model, it generates some artifacts. Curiously, these artifacts mimic some in vitro models found in the literature and raises the debate about the biological relevance of some conclusions that have been made in a fast growing field.