Untitled

doi:10.13040/ijpsr.0975-8232.5(11).4741-50

Cited by 1 publication

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the mean level of %T DNA was 22.7 in the cows from group 1, which was significantly higher than the level in the control group (3.5) and the results presented by Wójcik et al [129], which may indicate that the BVD virus negatively affected the DNA integrity. Other researchers have used the SCGE assay to assess the degree of genotoxicity and mutagenicity of the physical and chemical factors and observed a high frequency of DNA damage [39,[130][131][132][133][134][135]. This assay has also been used to assess the degree of DNA fragmentation resulting from the clastogenic effect of the papilloma virus Bovine papillomavirus [136], and it revealed an increased level of damage to genetic material.…”

Section: Discussionmentioning

confidence: 99%

Identification of Genomic Instability in Cows Infected with BVD Virus

Kępka,

Wójcik,

Wysokińska

2023

Animals

View full text Add to dashboard Cite

An important factor for dairy cattle farmers is the profitability of cattle rearing, which is influenced by the animals’ health and reproductive parameters, as well as their genomic stability and integrity. Bovine viral diarrhea (BVD) negatively affects the health of dairy cattle and causes reproductive problems. The aim of the study was to identify genomic instability in cows with reproductive disorders following infection with the BVD virus. The material for analysis was peripheral blood from Holstein-Friesian cows with reproductive problems, which had tested positive for BVD, and from healthy cows with no reproductive problems, which had tested negative for BVD. Three cytogenetic tests were used: the sister chromatid exchange assay, fragile sites assay, and comet assay. Statistically significant differences were noted between the groups and between the individual cows in the average frequency of damage. The assays were good biomarkers of genomic stability and enabled the identification of individuals with an increased frequency of damage to genetic material that posed a negative impact on their health. The assays can be used to prevent disease during its course and evaluate the genetic resistance of animals. This is especially important for the breeder, both for economic and breeding reasons. Of the three assays, the comet assay proved to be the most sensitive for identifying DNA damage in the animals.

show abstract