915 Development of Novel Optical Technologies for Early Colon Carcinogenesis Detection via Nanoscale Mass Density Fluctuations: Potential Implications for Endoscopic Diagnostics

Bajaj, Shailesh; Jing, Yi; Radosevich, Andrew J.; Goldberg, Michael J.; Rogers, Jeremy D.; Bianchi, Laura; Yen, Eugene F.; Upadhye, Sudeep; Tsang, Tat-Kin; Parker, Beth A.; Roy, Hemant K.; Backman, Vadim

doi:10.1016/j.gie.2012.04.146

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we use inverse spectroscopic optical coherence tomography (ISOCT) [23][24][25][26] to provide a quantification of the ultrastructural alterations in epithelium and stroma in field carcinogenesis. Optical coherence tomography (OCT) conventionally has been used to provide the microscopic reconstruction of tissue morphology with a spatial resolution on the order of several microns.…”

Section: Introductionmentioning

confidence: 99%

Spatially resolved optical and ultrastructural properties of colorectal and pancreatic field carcinogenesis observed by inverse spectroscopic optical coherence tomography

Radosevich

Stypula-Cyrus

et al. 2014

J. Biomed. Opt

Self Cite

View full text Add to dashboard Cite

Field carcinogenesis is the initial stage of cancer progression. Understanding field carcinogenesis is valuable for both cancer biology and clinical medicine. Here, we used inverse spectroscopic optical coherence tomography to study colorectal cancer (CRC) and pancreatic cancer (PC) field carcinogenesis. Depth-resolved optical and ultrastructural properties of the mucosa were quantified from histologically normal rectal biopsies from patients with and without colon adenomas (n=85) as well as from histologically normal peri-ampullary duodenal biopsies from patients with and without PC (n=22). Changes in the epithelium and stroma in CRC field carcinogenesis were separately quantified. In both compartments, optical and ultra-structural alterations were consistent. Optical alterations included lower backscattering (μb) and reduced scattering (μs') coefficients and higher anisotropy factor g. Ultrastructurally pronounced alterations were observed at length scales up to ∼450 nm, with the shape of the mass density correlation function having a higher shape factor D, thus implying a shift to larger length scales. Similar alterations were found in the PC field carcinogenesis despite the difference in genetic pathways and etiologies. We further verified that the chromatin clumping in epithelial cells and collagen cross-linking caused D to increase in vitro and could be among the mechanisms responsible for the observed changes in epithelium and stroma, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%