[91] 2-Keto-3-deoxy-6-phosphogluconic aldolase (crystalline)

Meloche, H. Paul; Ingram, J. M.; Wood, William F.

doi:10.1016/0076-6879(66)09103-1

Cited by 29 publications

(12 citation statements)

References 5 publications

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“…Chemicals. 2-Keto 3-deoxy 6-phosphogluconate aldolase was prepared from Pseudomonas putida PRS2015 by the method of Meloche et al (15). The first (NH4)2SO4 precipitate was used as the source of the enzyme.…”

Section: Materils and Methodsmentioning

confidence: 99%

Alginic acid synthesis in Pseudomonas aeruginosa mutants defective in carbohydrate metabolism

Banerjee¹,

Vanags²,

Chakrabarty³

et al. 1983

J Bacteriol

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Mutant cells of mucoid Pseudomonas aeruginosa isolated from cystic fibrosis patients were examined for their ability to synthesize alginic acid in resting cell suspensions. Unlike the wild-type strain which synthesizes alginic acid from glycerol, fructose, mannitol, glucose, gluconate, glutamate, or succinate, mutants lacking specific enzymes of carbohydrate metabolism are uniquely impaired. A phosphoglucose isomerase mutant did not synthesize the polysaccharide from mannitol, nor did a glucose 6-phosphate dehydrogenase mutant synthesize the polysaccharide from mannitol or glucose. Mutants lacking the Entner-Doudoroff pathway dehydrase or aldolase failed to produce alginate from mannitol, glucose, or gluconate, as a 3-phosphoglycerate kinase or glyceraldehyde 3-phosphate dehydrogenase mutant failed to produce from glutamate or succinate. These results demonstrate the primary role of the Entner-Doudoroff pathway enzymes in the synthesis of alginate from glucose, mannitol, or gluconate and the role of glyceraldehyde 3-phosphate dehydrogenase reaction for the synthesis from gluconeogenic precursors such as glutamate. The virtual absence of any activity of phosphomannose isomerase in cell extracts of several independent mucoid bacteria and the impairment of alginate synthesis from mannitol in mutants lacking phosphoglucose isomerase or glucose 6-phosphate dehydrogenase rule out free mannose 6-phosphate as an intermediate in alginate biosynthesis. grown overnight in the synthetic medium described by 238 on August 4, 2020 by guest

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Section: Materils and Methodsmentioning

confidence: 99%

Alginic acid synthesis in Pseudomonas aeruginosa mutants defective in carbohydrate metabolism

Banerjee¹,

Vanags²,

Chakrabarty³

et al. 1983

J Bacteriol

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“…6-Phosphogluconate dehydratase and 2-keto-3-deoxy-6phosphogluconate (KDPG) aldolase, the two enzymes comprising the pathway (see Figure 1), have been characterized extensively in P. putida by W. A. Wood and his coworkers (64,65,83,84). Kersters & De Ley have examined the distribution of these enzymes in a variety of gram-negative bacteria (61,62).…”

Section: Central Role Of the Entner-doudoroff Pathwaymentioning

confidence: 99%

Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Lessie¹,

Phibbs²

1984

Annu. Rev. Microbiol.

307

142

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“…Sucrose phosphorylase (EC 2.4.1 .7) was assayed in cell-free preparations by the method of Doudoroff (1955) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) by that of DeMoss (1955). 6-Phosphogluconate dehydratase (EC 4.2.1 .12) and 2-keto-3-deoxy-6-phosphogluconate aldolase (EC 4.1 .2.14) were identified in extracts by the method of MacGee & Doudoroff (1954) and were also tested individually as described by Meloche & Wood (1966) and Meloche et al (1966). Mannose isomerase (EC 5.3 .…”

Section: E T H O D Smentioning

confidence: 99%

Isolation and Properties of a New Hydrogen Bacterium Related to Pseudomonas saccharophila

Palleroni

1980

Microbiology

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[91] 2-Keto-3-deoxy-6-phosphogluconic aldolase (crystalline)

Cited by 29 publications

References 5 publications

Alginic acid synthesis in Pseudomonas aeruginosa mutants defective in carbohydrate metabolism

Alginic acid synthesis in Pseudomonas aeruginosa mutants defective in carbohydrate metabolism

Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Isolation and Properties of a New Hydrogen Bacterium Related to Pseudomonas saccharophila

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