90 Uptake of putrescine by <i>Trypanosoma granulosum</i>

Mastri, Calli; Rytlewski, Francesca; Thorborn, D.; Davies, Angela J.; Sappal, G.K.

doi:10.1042/bst026s376

Cited by 2 publications

(4 citation statements)

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“…In addition, a diamine uptake system similar to that in T. cruzi epimastigotes has been characterized (18). Therefore, T. granulosum appears similar to T. cruzi (4,5,19), but markedly different from African trypanosomes (20) and Leishmania (21,22) in lacking a functional ODC and relying on uptake of exogenous polyamines (18). The present study confirms these observations by examining polyamine and thiol metabolism in T. granulosum grown in vitro.…”

supporting

confidence: 86%

“…Indeed, the growth media contains sufficient polyamines to account for intracellular levels in both trypanosomes. Moreover, both organisms have been shown to possess similar uptake systems for putrescine (7,18). …”

Section: Discussionmentioning

confidence: 97%

“…It was found that very high concentrations of DFMO were required to inhibit growth (IC 50 ϭ 16 mM). In addition, a diamine uptake system similar to that in T. cruzi epimastigotes has been characterized (18). Therefore, T. granulosum appears similar to T. cruzi (4,5,19), but markedly different from African trypanosomes (20) and Leishmania (21,22) in lacking a functional ODC and relying on uptake of exogenous polyamines (18).…”

mentioning

confidence: 97%

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Polyamine and Thiol Metabolism in Trypanosoma granulosum: Similarities with Trypanosoma cruzi

Mastri

Thorborn

Davies

et al. 2001

Biochemical and Biophysical Research Communications

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supporting

confidence: 86%

Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 97%

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Polyamine and Thiol Metabolism in Trypanosoma granulosum: Similarities with Trypanosoma cruzi

Mastri

Thorborn

Davies

et al. 2001

Biochemical and Biophysical Research Communications

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“…Many cells, including ir: granulosum, have specific uptake systems for polyamines (Seiler & Dezeure 1990;Mastri, Rytlewski, Thorborn, Davies & Sappal 1998), while the enzymes ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAM-D) play a crucial role in controlling intracellular polyamine levels through biosynthesis. To further investigate the effects of polyamine biosynthesis inhibitors on the morphology of culture forms of T, panulosum, the ultrastructure of control organisms and those exposed to various concentrations of these drugs was studied.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of culture forms of the eel trypanosome, Trypanosoma granulosum Laveran & Mesnil, 1902, exposed to polyamine biosynthesis inhibitors

Davies

Thorborn²,

Mastri

et al. 1999

Journal of Fish Diseases

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Polyamines are important in the growth, division and differentiation of many cell types, including trypanosomes. The ultrastructure of culture forms of Trypanosoma granulosum was examined following growth in a modified semidefined medium (controls) and in the same medium with added polyamine biosynthesis inhibitors (DFMO, MGBG and Berenil). Untreated trypanosomes had ultrastructural features in common with other cultured flagellates. Those treated with DFMO were generally more rounded in contour. Cytoplasmic vacuoles and swollen mitochondrial membranes suggested osmotic imbalance, perhaps resulting from compromised membrane integrity. An anticlockwise arm dividing subtubule B of the peripheral doublets of the flagellum was noted in some trypanosomes treated with 20 mM DFMO. Vacuolation was also induced by MGBG and flagellar division occurred more frequently at 0.2 mM than in controls. With 1 mM MGBG, mitochondria were difficult to discern and kinetoplasts were disrupted. With 0.2 mM Berenil, vacuoles, swollen mitochondria, membranous whorls, additional microtubules underlying subpellicular tubules and disaggregated kinetoplasts were noted. With 1 mM Berenil, much of the cell structure was destroyed, although the pellicle, flagellum and paraxial rod remained intact. The present study illustrates the importance of hnctional polyamine synthetic pathways for the integrity of membranes, mitochondria, kinetoplasts and possibly microtubules in cultured T. granulosum.

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90 Uptake of putrescine by Trypanosoma granulosum

Cited by 2 publications

References 3 publications

Polyamine and Thiol Metabolism in Trypanosoma granulosum: Similarities with Trypanosoma cruzi

Polyamine and Thiol Metabolism in Trypanosoma granulosum: Similarities with Trypanosoma cruzi

Ultrastructure of culture forms of the eel trypanosome, Trypanosoma granulosum Laveran & Mesnil, 1902, exposed to polyamine biosynthesis inhibitors

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