9-Methylfascaplysin exerts anti-ischemic stroke neuroprotective effects via the inhibition of neuroinflammation and oxidative stress in rats

Zhang, Difan; Yi, Feng; Pan, Hanbo; Xuan, Zhenquan; Yan, Sicheng; Mao, Yuechun; Xiao, Xiao; Huang, Xinghan; Zhang, Hui; Fei, Zhou; Chen, Bojun; Chen, Xiaowei; Líu, Hao; Yan, Xiaojun; Liang, Hongze; Cui, Wei

doi:10.1016/j.intimp.2021.107656

Cited by 10 publications

(3 citation statements)

References 41 publications

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“…The mice were sacrificed for RT-qPCR analysis. Total RNA was extracted from HDB cells using TRIzol reagent (Thermo Fisher, Waltham, MA, United States; Zhang et al, 2021 ). Glyceraldehyde-3-phosphate dehydrogenase (GADPH) and β-actin mRNA levels were used as controls.…”

Section: Methodsmentioning

confidence: 99%

Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons

Zhou

Zhang

Wang

et al. 2023

Front. Aging Neurosci.

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Background and objectivesDepression is a common comorbidity of dementia and may be a risk factor for dementia. Accumulating evidence has suggested that the cholinergic system plays a central role in dementia and depression, and the loss of cholinergic neurons is associated with memory decline in aging and Alzheimer’s patients. A specific loss of cholinergic neurons in the horizontal limb of the diagonal band of Broca (HDB) is correlated with depression and dysfunction of cognition in mice. In this study, we examined the potential regenerative mechanisms of knockdown the RNA-binding protein polypyrimidine tract binding protein (PTB) in reversing depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons.MethodsWe lesioned cholinergic neurons in mice induced by injection of 192 IgG-saporin into HDB; then, we injected either antisense oligonucleotides or adeno-associated virus-shRNA (GFAP promoter) into the injured area of HDB to deplete PTB followed by a broad range of methodologies including behavioral examinations, Western blot, RT-qPCR and immunofluorescence.ResultsWe found that the conversion of astrocytes to newborn neurons by using antisense oligonucleotides on PTB in vitro, and depletion of PTB using either antisense oligonucleotides or adeno-associated virus-shRNA into the injured area of HDB could specifically transform astrocytes into cholinergic neurons. Meanwhile, knockdown of PTB by both approaches could relieve the depression-like behaviors shown by sucrose preference, forced swimming or tail-suspension tests, and alleviate cognitive impairment such as fear conditioning and novel object recognition in mice with lesioned cholinergic neurons.ConclusionThese findings suggest that supplementing cholinergic neurons after PTB knockdown may be a promising therapeutic strategy to revert depression-like behaviors and cognitive impairment.

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Section: Methodsmentioning

confidence: 99%

Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons

Zhou

Zhang

Wang

et al. 2023

Front. Aging Neurosci.

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“…[50][51][52] Furthermore, a neuroinflammatory response may also involve the secretion of chemokines including CCL2, 53 CCL3, 54 CCL5, 55 CCL7, 56 CCL12, 57 among others, 58,59 second messengers (Nitric oxide), 60 and reactive species of oxygen. 61,62 These molecules promote the recruitment of other immune cells like macrophages, 63 DCs, 64 neutrophils, 65 eosinophils, 66 T cells, 52,67,68 and B cells 69 circulating in nonparenchymal areas, like meninges, choroid plexus, and perivascular space to participate the neuroinflammatory response. [70][71][72] Under homeostasis, leukocytes are present but are scarce in the CNS 73 ; however, increased numbers of CNS-infiltrated leukocytes have been identified under pathological conditions, 69,73,74 and their functions have been related to neuronal pathologies.…”

Section: Neuroinflammationmentioning

confidence: 99%

“…Activated microglia induce the inflammatory response in nervous tissue (neuroinflammation) by the secretion of pro‐inflammatory cytokines, such as IL‐1β, IL‐6, interferon gamma (IFN‐γ), and tumor necrosis factor alpha (TNF‐α) 50–52 . Furthermore, a neuroinflammatory response may also involve the secretion of chemokines including CCL2, 53 CCL3, 54 CCL5, 55 CCL7, 56 CCL12, 57 among others, 58,59 second messengers (Nitric oxide), 60 and reactive species of oxygen 61,62 63 DCs, 64 neutrophils, 65 eosinophils, 66 T cells, 52,67,68 and B cells 69 circulating in non‐parenchymal areas, like meninges, choroid plexus, and perivascular space to participate the neuroinflammatory response 70–72 .…”

Section: Neuroinflammationmentioning

confidence: 99%

The meningeal lymphatic vasculature in neuroinflammation

et al. 2022

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The lymphatic vasculature is a unidirectional network of lymphatic endothelial cells, whose main role is to maintain fluid homeostasis along with the absorption of dietary fat in the gastrointestinal organs and management and coordination of immune cell trafficking into lymph nodes during homeostasis and under inflammatory conditions. In homeostatic conditions, immune cells, such as dendritic cells, macrophages, or T cells can enter into the lymphatic vasculature and move easily through the lymph reaching secondary lymph nodes where immune cell activation or peripheral tolerance can be modulated. However, under inflammatory conditions such as pathogen infection, increased permeabilization of lymphatic vessels allows faster immune cell migration into inflamed tissues following a chemokine gradient, facilitating pathogen clearance and the resolution of inflammation. Interestingly, since the re‐discovery of lymphatic vasculature in the central nervous system, known as the meningeal lymphatic vasculature, the role of these lymphatics as a key player in several neurological disorders has been described, with emphasis on the neurodegenerative process. Alternatively, less has been discussed about meningeal lymphatics and its role in neuroinflammation. In this review, we discuss current knowledge about the anatomy and function of the meningeal lymphatic vasculature and specifically analyze its contribution to different neuroinflammatory processes, highlighting the potential therapeutic target of meningeal lymphatic vasculature in these pathological conditions.

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Derivatization of Marine‐Derived Fascaplysin via Highly Regioselective Suzuki‐Miyaura Coupling Contributing to the Enhanced Antibacterial Activity

et al. 2022

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A new methodology for the synthesis of fascaplysin derivatives has been established. A library of diversified fascaplysins can be efficiently and quickly prepared by regioselective Suzuki‐Miyaura coupling and subsequent quaternization for the first time. The minimum inhibitory concentration assessment showed that the unique 5‐ring coplanar aromatic system plays a key role in the resulting bioactivity, and functional groups at different substitution position exert differentiable impact on the bactericidal activity. some synthesized fascaplysin derivatives exhibited higher antibacterial activities than the pristine fascaplysin and the positive controls, especially against Gram‐negative bacteria Escherichia coli (ATCC 25922). These novel fascaplysin derivatives may have promising application potentials as bactericidal drugs.

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9-Methylfascaplysin exerts anti-ischemic stroke neuroprotective effects via the inhibition of neuroinflammation and oxidative stress in rats

Cited by 10 publications

References 41 publications

Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons

Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons

The meningeal lymphatic vasculature in neuroinflammation

Derivatization of Marine‐Derived Fascaplysin via Highly Regioselective Suzuki‐Miyaura Coupling Contributing to the Enhanced Antibacterial Activity

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