9-AAA inhibits growth and induces apoptosis in human melanoma A375 and rat prostate adenocarcinoma AT-2 and Mat-LyLu cell lines but does not affect the growth and viability of normal fibroblasts

Abstract

Abstract. The present study found that, similarly to 5-f luorouracil, low concentrations (1-10 µM) of 9-aminoacridine (9-AAA) inhibited the growth of the two rat prostate cancer AT-2 and Mat-LyLu cell lines and the human melanoma A375 cell line. However, at the same concentrations, 9-AAA had no effect on the growth and apoptosis of normal human skin fibroblasts (HSFs). The differences between the cellular responses of the AT-2 and Mat-LyLu cell lines, which differ in malignancy, were found to be relatively small compared with the differences between normal HSFs and the cancer cell lines. Visible effects on the cell growth and survival of tumor cell lines were observed after 24-48 h of treatment with 9-AAA, and increased over time. The inhibition of cancer cell growth was found to be due to the gradually increasing number of cells dying by apoptosis, which was observed using two methods, direct counting and FlowSight analysis. Simultaneously, cell motile activity decreased to the same degree in cancer and normal cells within the first 8 h of incubation in the presence of 9-AAA. The results presented in the current study suggest that short-lasting tests for potential anticancer substances can be insufficient; which may result in cell type-dependent differences in the responses of cells to tested compounds that act with a delay being overlooked. The observed differences in responses between normal human fibroblasts and cancer cells to 9-AAA show the requirement for additional studies to be performed simultaneously on differently reacting cancer and normal cells, to determine the molecular mechanisms responsible for these differences.

Explant-Like Passaging of Cells Growing on Portable Substrata Permits the Avoidance of Enzyme Application and Facilitates the Passage Procedure

“…Experiments were performed on rat 3T3 fibroblasts, AT-2 rat prostate adenocarcinoma cells of the Dunning R3327 series cell line , normal mouse astrocytes C8-D1A, and human glioblastoma multiforme T98G cells. Before experimentation, cells were cultured in 25 cm 2 Falcon dishes at 37 o C and 5% CO 2 while maintaining high humidity as described previously (MUSIALIK et al 2013;KOROHODA et al 2016;PUDE£EK et al 2019;RYSZAWY et al 2019;RYSZAWY et al 2020). AT-2 and C8-D1A cells were grown in RPMI-1640 medium (Sigma).…”