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Carballo, José Luís; Hernández-Inda, Zaira L; Pérez, Pilar; Garcia‐Gravalos, M. D.

doi:10.1186/1472-6750-2-17

Cited by 272 publications

(94 citation statements)

References 20 publications

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“…It indicates that the lethality effect of leaf extract is time dependent, increasing significantly after 24 h exposure. At this time nauplii are mainly in instar II/III [4] .…”

Section: Discussionmentioning

confidence: 89%

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In vivo toxicity study of Lantana camara

Pour

Sasidharan

2011

Asian Pacific Journal of Tropical Biomedicine

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“…It indicates that the lethality effect of leaf extract is time dependent, increasing significantly after 24 h exposure. At this time nauplii are mainly in instar II/III [4] .…”

Section: Discussionmentioning

confidence: 89%

“…It is used as a "benchtop bioassay" for the discovery and purification of bioactive natural products and is an excellent choice for elementary toxicity investigations of consumer products. The shrimp lethality assay is based on the ability to kill laboratory-cultured Artemia nauplii (animal's eggs) [4] . …”

Section: Introductionmentioning

confidence: 99%

In vivo toxicity study of Lantana camara

Pour

Sasidharan

2011

Asian Pacific Journal of Tropical Biomedicine

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“…Each treatment and control sample was distributed in triplicate into the sterile petri-dish before incubation at 28 °C under constant illumination in a digital incubator (MRC Laboratory Equipment, Model LE-509). Thereafter, the petri-dishes were examined with the aid of a hand lens against a white background that allows the moving larvae (naupili) to be separated from shells and counted at every 12 h for 72 h. The percentage hatchability was calculated by comparing the number of hatched naupili with the total number of brine shrimp eggs stocked (Carballo et al, 2002).…”

Section: Hatchability Test Of Brine Shrimpsmentioning

confidence: 99%

Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds

Kayode

Afolayan

2015

J. Zhejiang Univ. Sci. B

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Abstract:Renewed interest in natural materials as food flavors and preservatives has led to the search for suitable essential oils. Moringa oleifera seed essential oil was extracted by solvent-free microwave and hydrodistillation. This study assessed its chemical constituents. Cytotoxicity of the oils was investigated using hatchability and lethality tests on brine shrimps. A total of 16 and 26 compounds were isolated from the hydrodistillation extraction (HDE) and solvent-free microwave extraction (SME) oils, respectively, which accounted for 97.515% and 97.816% of total identifiable constituents, respectively. At 24 h when the most eggs had hatched, values of the SME (56.7%) and HDE (60.0%) oils were significantly different (P<0.05) from those of sea water (63.3%) and chloramphenicol (15.0%). Larva lethality was different significantly (P<0.05) between HDE and SME oils at different concentrations and incubation periods. The median lethal concentration (LC 50 ) of the oils was >1000 mg/ml recommended as an index for non-toxicity, which gives the oil advantage over some antioxidant, antimicrobial, therapeutic, and preservative chemicals.

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“…35 Results are given in Table 2 for the compounds which exhibited optimal cytotoxic effect against cancer cell lines, being the eight compounds 2b, 6, 11b, 11c, 12b, 16a, 16b and 18a. The shrimp lethality assay is considered as a useful tool for preliminary assessment of toxicity, and it has been used for the detection of fungal toxins, plant extract toxicity, heavy metals, cyano bacteria toxins, pesticides, and cytotoxicity testing of dental materials, 36 natural and synthetic organic compounds. 34 It has also been shown that A. salina toxicity test results have a correlation with rodent and human acute oral toxicity data.…”

Section: Toxicitymentioning

confidence: 99%

New approaches for the synthesis, cytotoxicity and toxicity of heterocyclic compounds derived from 2-cyanomethyl benzo[c]imidazole

Mohareb

Mohamed²,

Abdallah³

2016

ACSi

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The reaction of ethyl cyanoacetate with o-phenylenediamine gave the 2-cyanomethylbenzo[c]imidazole (1). The latter compound was used as the key starting material to synthesise biologically active heterocyclic derivatives. Thus, the reaction of 1 with cyclohexanone and either of benzaldehyde, 4-methoxybenzaldehyde or 4-chlorobenzaldehyde gave the annulated derivatives 2a-c, respectively. The antitumor evaluations of the newly synthesized products against the three cancer cell lines MCF-7 (breast adeno-carcinoma), NCI-H460 (non-small cell lung cancer) and SF-268 (CNS cancer) showed that compounds 2b, 6, 11b, 11c, 12b, 16a, 16b and 18a exhibited optimal cytotoxic effect against cancer cell lines, with IC 50 values in the nM range. Bioactive compounds are often toxic to shrimp larvae. Thus, in order to monitor these chemicals in vivo lethality to shrimp larvae (Artemia salina), Brine-Shrimp Lethality Assay was used. Compounds 11b, 12b and 16b showed no toxicity against the tested organisms.

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Untitled

Cited by 272 publications

References 20 publications

In vivo toxicity study of Lantana camara

In vivo toxicity study of Lantana camara

Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds

New approaches for the synthesis, cytotoxicity and toxicity of heterocyclic compounds derived from 2-cyanomethyl benzo[c]imidazole

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