89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?

Abstract

BackgroundThe exact mechanism of systemic hypersensitivity to venom is not exactly understood. It is suggested T cells with regulatory potential can downregulate other T cell subsets and effector cells, ex. mast cell or basophils. We focused on relationship of specific basophil reactivity in relationship to proportion of regulatory T cells.MethodsForty-five patients with history of systemic symptoms of allergy to Hymenoptera venom were included. Basophil reactivity before the treatment and after one year of allergen immunotherapy (AIT) was measured by CD63 expression, CD203c marker used for basophil identification. Cells were stimulated by aqueous solution of allergen in concentration range 0.01 to 1 μg/mL. T regulatory cells were identified as CD4 positive, CD25 bright and CD127 negative at the same interval of treatment. Monoclonal antibodies conjugated with fluorochromes were used, measured by FACSCalibur. Paired t test and correlation analysis used for statistical evaluation.ResultsMedian Treg proportion before therapy was 2.160, after IT 1.960, basophil specific response (proportion of CD63 positive cells) at the same interval were 3.65 and 4.11 at 0.01 μg/mL, 13.1 and 16.1 for 0.1 μg/mL and 33.85 and 40.8 for 1 μg/mL. All differences were not statistically significant. Differences of basophil activation were not significantly related to proportion of T regulatory cells.ConclusionsIn our group of patients with HV allergy, treated by AIT, we did not found any relationship between basophil specific activation during allergen immunotherapy and proportion of T regulatory cells.

Prueba de activación de basófilos en el seguimiento de pacientes que reciben inmunoterapia con veneno de himenópteros: revisión de la evidencia actual

“…58 Algunos estudios señalan que los pacientes que no toleraban la repicadura, después de un periodo prolongado de inmunoterapia, tenían valores más altos de basófilos CD63 por el estímulo específico que los no respondedores. 70,71 Otro estudio informó que la liberación de histamina fue mayor en pacientes que recibieron inmunoterapia con veneno de abeja, que reaccionaban con la prueba de repicadura realizada al finalizar la inmunoterapia, en comparación con los tolerantes. 72 Todos estos estudios apoyan la indi-cación de la prueba de activación de basófilos como biomarcador útil para vigilar la eficacia de la inmunoterapia con veneno de himenópteros 20 y sugiere que deben valorarse las concentraciones que produce una respuesta submáxima, puesto que los cambios en la respuesta máxima (reactividad) pueden no aportar información del estado clínico de tolerancia, especialmente en las fases iniciales del tratamiento.…”