[86] Measurement of antagonistic effects of growth factors and interferons

Inglot, Anna D.; Pajtasz, E

doi:10.1016/0076-6879(86)19088-4

Cited by 4 publications

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Interactions of interferons and transforming growth factors during clonal growth of mouse or human cells in soft agar and in mice

Popik

Inglot

1987

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

The mouse fibroblast-like transformed cell line C-243 adapted to growth in suspension was used as a source of virus-induced interferons (MulFN-alpha, beta), and spontaneously produced active growth factors. These factors were purified from C-243 cells grown as tumors in BALB/c mice, and had properties identical to those of TGF-alpha or TGF-beta isolated by others from different tissues. Exogenous TGF-alpha, beta stimulated colony formation by C-243 cells in soft agar, whereas MulFN-alpha, beta inhibited it. Clonal growth of human lung adenocarcinoma A549 cells in soft agar was inhibited as well by human interferons (types alpha, beta, or gamma) as by TGF-beta. Inhibition was dose-related. Pure EGF, which is an analogue of TGF-alpha, diminished the antiproliferative activity of interferons alpha, beta, and gamma in A549 cells. On the other hand, the anti-mitogenic action of IFN-beta and TGF-beta was clearly synergistic. In mice bearing C-243 cell tumors, TGF-alpha, beta stimulated growth, whereas MulFN-alpha, beta inhibited it. Stimulation of tumor growth was also observed after administration of anti-IFN serum that could neutralize endogenous IFN-alpha, beta. The simultaneous administration of MulFN-alpha, beta and TGF-alpha, beta diminished anti-tumor effects of IFN in mice. Our results suggest that both TGFs and IFNs are autocrine, positive or negative growth factors modulating the rate of proliferation and the neoplastic behavior of the cells. The final effects depend on the target-cell sensitivity and on the relative concentration of the various hormone-like factors. Cancer cells overstimulated by TGF-alpha, beta or by EGF may not respond to IFNs.

show abstract

Interactions of interferons and transforming growth factors during clonal growth of mouse or human cells in soft agar and in mice

Popik

Inglot

1987

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

show abstract

The Inhibitory Effects of Growth Factors and Cytokines on Cell Proliferation

Goldstein¹,

Wilding²

1993

Humoral Factors in the Regulation of Tissue Growth

View full text Add to dashboard Cite

Transforming Growth Factor-μ Inhibits the Antiviral Action of Interferons in Human Embryonic Fibroblasts

Zielińska-Jenczylik

Inglot²,

Popik³

1991

Journal of Interferon Research

View full text Add to dashboard Cite

Transforming growth factor-beta (TGF-beta) at concentration of 10 ng/ml inhibited the development of the interferon-alpha- (IFN-alpha) or IFN-gamma-induced antiviral state in quiescent human embryonic fibroblasts. The action of the cytokines was dose-related; TGF-beta had no direct effect on the replication of either vesicular stomatitis virus (VSV) or encephalomyocarditis virus (EMCV) used as the challenge viruses in the IFN assays. We suggest that despite the fact that TGF-beta acts mainly as a "negative" growth factor, its interactions with IFNs in the antiviral assays resemble known effects of the typical "positive" peptide growth factors.

show abstract

[86] Measurement of antagonistic effects of growth factors and interferons

Cited by 4 publications

References 8 publications

Interactions of interferons and transforming growth factors during clonal growth of mouse or human cells in soft agar and in mice

Interactions of interferons and transforming growth factors during clonal growth of mouse or human cells in soft agar and in mice

The Inhibitory Effects of Growth Factors and Cytokines on Cell Proliferation

Transforming Growth Factor-μ Inhibits the Antiviral Action of Interferons in Human Embryonic Fibroblasts

Contact Info

Product

Resources

About