Abstract:Use of frozen semen by the horse industry is popular. Several extenders have been used to freeze stallion semen; the most used are EDTA-lactose egg yolk and INRA 82. A new extender named Botu-Crio� (BioTech, Botucatu, Sao Paulo, Brazil) has been used on a large scale in Brazil. No comparisons of these extenders have been published. The present experiment was designed to compare the ability of these three extenders to preserve sperm longevity after incubation. One ejaculate from each of 13 stallions was used. A… Show more
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