83. Histamine release from rat mast cells by antibodies against IgE-receptors

Ishizaka, Kimishige; Conrad, Dan; Froese, Arnold

doi:10.1016/0091-6749(78)90322-6

Cited by 39 publications

(49 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cellular activation by anti-receptor antibody has previously been observed in several systems (26)(27)(28)(29)(30). In these examples a single application of a polyclonal or monoclonal antibody was effective, whereas in our studies (Fig.…”

Section: Discussionmentioning

confidence: 96%

Human platelet Fc receptor for immunoglobulin G. Identification as a 40,000-molecular-weight membrane protein shared by monocytes.

Rosenfeld¹,

Looney²,

Leddy³

et al. 1985

J. Clin. Invest.

224

View full text Add to dashboard Cite

We have recently shown that human monocytes and U937 cells possess two molecular classes of Fcy receptor. One, a 72,000-mol-wt sialoglycoprotein, has high affinity for certain subclasses of human and murine monomeric IgG. The other is a 40,000-mol-wt protein (p40) with low affinity for monomeric IgG but with the capacity to bind IgG aggregates or IgG-coated particles.In the present study, a 40,000-mol-wt single chain protein, apparently identical to p40 from U937 cells, was isolated from surface-radioiodinated human platelets by affinity purification using a murine IgG2b monoclonal antibody to p40. This 40,000-mol-wt protein was not seen when control IgG2b or unrelated murine monoclonal antibodies were employed in place of antip40. The same 40,000-mol-wt protein was also recovered from an IgG-Sepharose affinity adsorbent, but not from ovalbuminor myoglobin-Sepharose. The 72,000-mol-wt Fcvy receptor of monocytes was not identified on platelets.Monoclonal anti-p4O and Fab fragments derived from this antibody blocked platelet aggregation by heat-aggregated human IgG, whereas a control murine IgG2b protein had little or no inhibitory effect at 500-1,000-fold higher concentrations. A murine IgG1 monoclonal antibody, reactive with an unrelated platelet-specific membrane antigen, did not inhibit platelet responses to aggregated IgG. Anti-p4O did not affect platelet aggregation by thrombin, collagen, or fibrinogen plus ADP. Although antip40 did not directly aggregate platelets in the concentrations employed, cross-linking with F(ab'`) goat anti-murine Ig induced apyrase-sensitive aggregation of anti-p40-treated platelets. This indicates that p40 possesses transmembrane linkage for platelet activation and secretion.These observations strongly suggest that this newly recognized 40,000-mol-wt platelet membrane protein serves as an Fcy receptor.

show abstract

Section: Discussionmentioning

confidence: 96%

Human platelet Fc receptor for immunoglobulin G. Identification as a 40,000-molecular-weight membrane protein shared by monocytes.

Rosenfeld¹,

Looney²,

Leddy³

et al. 1985

J. Clin. Invest.

224

View full text Add to dashboard Cite

show abstract

“…Evidence that FcR aggregation is a necessary and sufficient signal for triggering a cellular response (17)(18)(19)(20) was based on studies using multivalent ligands specific of a single FcR. Most cells, however, express several types of FcR and antibodies of more than one isotype are usually produced together against a given antigen.…”

Section: Introductionmentioning

confidence: 99%

Regulation of high-affinity IgE receptor-mediated mast cell activation by murine low-affinity IgG receptors.

Daëron¹,

Malbec²,

Latour³

et al. 1995

J. Clin. Invest.

332

225

View full text Add to dashboard Cite

show abstract

“…For granulocytes, such as neutrophils, eosinophils and mast cells, cross-linking FcγRs triggers rapid degranulation, and the generation and release of reactive oxygen and nitrogen intermediates with potent bactericidal activity (20,30,31,67,(69)(70)(71)(72)(73). Likewise, FcγRIIIa-cross-linking on NK cells triggers cellular activation and the release of enzymes, such as perforin and granzymes, from intracellular granules that induces the formation of pores at the cell membrane of IgGcoated cellular targets, leading ultimately to the activation of pro-apoptotic pathways and cell death (22,74).…”

Section: Diversity Of Fc Effector Functionsmentioning

confidence: 99%