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Svetleva, D.

doi:10.1023/a:1023983831582

Cited by 32 publications

(8 citation statements)

References 77 publications

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“…3 regarding adenine usage. Of course, this genotype-dependent response is also determined by the type or state of the explant, cell or tissue specialization and by the cultivation conditions 4 . Nevertheless, the common bean was regenerated via organogenic 5–7 and embryogenic pathways 8–10 from different plant explants: intact seedlings, embryonic axes, cotyledons, cotyledonary nodes, leaf petioles, internodes, auxiliary shoots, hypocotyls or leaves 3,11–13 .…”

Section: Introductionmentioning

confidence: 99%

“…The plant material included six varieties: Czerwona, Plus, Laponia, Złota Saxa, Ibiza and Goldpantera (the last four are listed in The Common Catalogue of Varieties of Vegetable Species , 35th complete edition, 2016) 14 . The studies were conducted on randomly chosen cultivars as a starting point, following Malmberg’s 15 suggestion, assuming that the wide screening of genetic lines might become a base of knowledge for plant regeneration 1,4 . It should be noted here that different parts of young and mature plants were tested in our research prephase (preliminary investigation of the regeneration capacity tested on a small plant pool, 20 explants, data not shown ).…”

Section: Introductionmentioning

confidence: 99%

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Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis

Hnatuszko-Konka

Kowalczyk

Gerszberg

et al. 2019

Sci Rep

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The tissue culture of Phaseolus vulgaris has always been considered difficult. Its regenerative capacity and response to culture conditions are highly genotype-dependent and hamper the application of genetic engineering. The objective of this study was to develop a repeatable technique for organogenic bud induction from selected explants of the common bean. Epicotyls and hypocotyls of six cultivars were investigated to determine the effect of the genotype, and four variants of two basal media (Murashige-Skoog and Gamborg) were tested. The composition of these medium variants was based on the published data suggesting the most universal medium compounds that show the advantage of being applicable to different cultivars. As a result, the common bean epicotyls showed undisputed regeneration superiority over the hypocotyls. Moreover, a well-known observation was confirmed, namely that common bean regeneration is cultivar-specific or at least specific to the cluster of related cultivars. However, efficient regeneration was achieved most often when the epicotyls were incubated on the MS or B5 media amended with AgNO 3 and BAP. Additionally, the positive synergistic influence of activated charcoal and silver nitrate on bud formation was demonstrated. The highest values of the epicotyl in vitro response for the common bean cultivars could be presented as follows: Czerwona (70.00%) > Goldpantera (58.89%) and Ibiza (58.89%) > Plus (55.56%) > Laponia (50.56%) > Złota Saxa (46.11%).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis

Hnatuszko-Konka

Kowalczyk

Gerszberg

et al. 2019

Sci Rep

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“…Genetic engineering is a powerful tool to introduce genes from sources that are inaccessible through sexual hybridization [12][13][14]. Thus, substantial efforts have been made to develop reliable transformation methods for engineering common beans with various traits [13,[15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.)

et al. 2020

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Stable transformation of common bean (Phaseolus vulgaris L.) has been successful, to date, only using biolistic-mediated transformation and shoot regeneration from meristemcontaining embryo axes. In this study, using precultured embryo axes, and optimal co-cultivation conditions resulted in a successful transformation of the common bean cultivar Olathe using Agrobacterium tumefaciens strain EHA105. Plant regeneration through somatic embryogenesis was attained through the preculture of embryo axes for 12 weeks using induced competent cells for A. tumefaciens-mediated gene delivery. Using A. tumefaciens at a low optical density (OD) of 0.1 at a wavelength of 600 nm for infection and 4-day co-cultivation, compared to OD 600 of 0.5, increased the survival rate of the inoculated explants from 23% to 45%. Selection using 0.5 mg L-1 glufosinate (GS) was effective to identify transformed cells when the bialaphos resistance (bar) gene under the constitutive 35S promoter was used as a selectable marker. After an 18-week selection period, 1.5%-2.5% inoculated explants, in three experiments with a total of 600 explants, produced GS-resistant plants through somatic embryogenesis. The expression of bar was confirmed in first-and secondgeneration seedlings of the two lines through reverse polymerase chain reaction. Presence of the bar gene was verified through genome sequencing of two selected transgenic lines. The induction of regenerable, competent cells is key for the successful transformation, and the protocols described may be useful for future transformation of additional Phaseolus germplasm.

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“…En Centroamérica, el frijol ayocote se cultiva casi exclusivamente en asociación con maíz (Zea mays L.) (Vargas-Vázquez et al, 2007). Dado que los frijoles son un elemento importante en el autoabasto de poblaciones rurales, además de ser la leguminosa de grano más importante constituye la fuente principal de proteína de calidad a bajo costo, con diversas variaciones en cuanto a formas de cultivo, uso y maneras de preparación para consumo humano (Svetleva et al, 2003).…”

Section: Introductionunclassified

ANÁLISIS DE LA ESTRUCTURA POBLACIONAL DEL FRIJOL AYOCOTE (Phaseolus coccineus L.) MEDIANTE AFLP.

et al. 2019

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RESUMEN: El frijol ayocote (Phaseolus coccineus L.; 2n=2x=22) es una leguminosa originaria de las zonas altas de México; es una especie que ha sido poco estudiada a pesar de su importancia para consumo humano y el uso potencial en el mejoramiento de la resistencia de Phaseolus a enfermedades y factores abióticos (tolerancia a bajas temperaturas). El objetivo del presente estudio fue determinar la estructura poblacional de 117 colectas de P. coccineus correspondientes a 15 localidades del Carso Huasteco del estado de Puebla, México, más cinco testigos para conocer el grado de variablidad genética existente en germoplasma criollo mexicano de P. coccineus mediante la técnica de polimorfismos en longitud de fragmentos amplificados (AFLP). La estructura poblacional indicó un valor delta K = 3. El polimorfismo fue del 87.4% y el AMOVA confirmó la baja diferenciación genética entre accesiones (F st = 0.067), la varianza explicada entre y dentro de poblaciones fue 6.7 y 93.3%, respectivamente. El análisis de conglomerados no mostró agrupamientos definidos con base en el origen del germoplasma.

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Cited by 32 publications

References 77 publications

Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis

Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis

Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.)

ANÁLISIS DE LA ESTRUCTURA POBLACIONAL DEL FRIJOL AYOCOTE (Phaseolus coccineus L.) MEDIANTE AFLP.

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