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Neville, Margaret C.; McFadden, T.B.; Forsyth, Isabel A.

doi:10.1023/a:1015770423167

Cited by 501 publications

(148 citation statements)

References 105 publications

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“…PRL (or placental lactogens) along with P4 are each necessary for lobuloalveolar development (5,6). Both are secreted at high levels during early and middle pregnancy, when lobuloalveolar growth is maximal.…”

Section: Figmentioning

confidence: 99%

Receptor Activator of NF-κB Ligand Induction via Jak2 and Stat5a in Mammary Epithelial Cells

Srivastava

Matsuda

Hou

et al. 2003

Journal of Biological Chemistry

115

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Prolactin (PRL) is the primary hormone that, in conjunction with local factors, leads to lobuloalveolar development during pregnancy. Recently, receptor activator of NF-B ligand (RANKL) has been identified as one of the effector molecules essential for lobuloalveolar development. The molecular mechanisms by which PRL may induce RANKL expression have not been carefully examined. Here we report that RANKL expression in the mammary gland is developmentally regulated and dependent on PRL and progesterone, whereas its receptor RANK (receptor activator of NF-B) and decoy receptor osteoprotegerin (OPG) are constitutively expressed at all stages in both normal (PRL ؉/؊ ) and prolactin knockout (PRL ؊/؊ ) mice. In vitro, PRL markedly increased RANKL expression in primary mammary epithelial cells and RANKL-luciferase reporter activity in CHOD6 cells, which constitutively express the PRL receptor. We identified a ␥-interferon activation sequence (GAS) in the region between residues ؊965 to ؊725 of the RANKL promoter, which conferred a PRL response. Using dominant negative mutants of recombinant Jak2 and Stat5 in CHOD6 cells, and by reconstituting the Jak2/Stat5 pathway in COS7 cells, we determined that Jak2 and Stat5a are essential for the PRL-induced RANKL expression in mammary gland.

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Section: Figmentioning

confidence: 99%

Receptor Activator of NF-κB Ligand Induction via Jak2 and Stat5a in Mammary Epithelial Cells

Srivastava

Matsuda

Hou

et al. 2003

Journal of Biological Chemistry

115

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“…All continued to increase in subsequent intervals falling into 4 related groups with slightly different patterns of expression. The one gene that does not increase during pregnancy (clustered to FFIF 75) is PTHrP, a gene encoding a protein hormone involved in calcium regulation (Neville et al, 2002), which could be deleterious in the pregnant animal and may, therefore be differentially regulated. The genes of energy metabolism, with two exceptions, fell into downward going clusters, suggesting a relative fall in ATP generation in this tissue, which must devote most of its energy to synthetic reactions and transport.…”

Section: Separation Of Functional Groups By Trajectory Clusteringmentioning

confidence: 99%

“…Data was acquired by Affymetrix oligonucleotide-based microarray data showing secretory activation in the mouse mammary gland. Secretory activation is a unidirectional process that takes place with high temporal coherence during the physiological transition from pregnancy to lactation (Neville et al, 2002). Many of the biochemical events in this process have been studied extensively for more than three decades (Wilde et al 1986;Mellenberger and Bauman, 1974;Kuhn, 1968), and it is clear that many of the changes are transcriptionally regulated (Rosen et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Trajectory Clustering: A Non-Parametric Method for Grouping Gene Expression Time Courses, With Applications to Mammary Development

et al. 2002

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Trajectory clustering is a novel and statistically well-founded method for clustering time series data from gene expression arrays. Trajectory clustering uses non-parametric statistics and is hence not sensitive to the particular distributions underlying gene expression data. Each cluster is clearly defined in terms of direction of change of expression for successive time points (its 'trajectory'), and therefore has easily appreciated biological meaning. Applying the method to a dataset from mouse mammary gland development, we demonstrate that it produces different clusters than Hierarchical, K-means, and Jackknife clustering methods, even when those methods are applied to differences between successive time points. Compared to all of the other methods, trajectory clustering was better able to match a manual clustering by a domain expert, and was better able to cluster groups of genes with known related functions.

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“…It is well known that levels of human glucuronidation gradually increase through development, including the weeks and months following birth. Yet it might be anticipated that the dramatic changes in the levels of circulating hormones that occur during pregnancy and lactation (24) may alter the levels of hepatic enzymes in maternal liver. In rodents, several studies indicate that maternal liver glucuronidation activity is lower during pregnancy (25,26).…”

mentioning

confidence: 99%

Tissue-specific, Inducible, and Hormonal Control of the Human UDP-Glucuronosyltransferase-1 (UGT1) Locus

Chen

Beaton

Nguyen

et al. 2005

Journal of Biological Chemistry

114

106

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The human UDP-glucuronosyltransferase 1 (UGT1) locus spans nearly 200 kb on chromosome 2 and encodes nine UGT1A proteins that play a prominent role in drug and xenobiotic metabolism. Transgenic UGT1 (Tg-UGT1) mice have been created, and it has been demonstrated that tissue-specific and xenobiotic receptor control of the UGT1A genes is influenced through circulating humoral factors. In Tg-UGT1 mice, the UGT1A proteins are differentially expressed in the liver and gastrointestinal tract. Gene expression profiles confirmed that all of the UGT1A genes can be targeted for regulation by the pregnane X receptor activator pregnenolone-16␣-carbonitrile (PCN) or the Ah receptor ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). In addition, the selective induction of glucuronidation activity toward lamotrigine, ethinyl estradiol, chenodeoxycholic acid, and lithocholic acid by either PCN or TCDD in small intestine from Tg-UGT1 mice corresponded to expression of the locus in this tissue. Induction of UGT1A1 by PCN and TCDD is believed to be highly dependent upon glucocorticoids, because submicromolar concentrations of dexamethasone actively promote PCN and TCDD induction of UGT1A1 in Tg-UGT1 primary hepatocytes. The role of hormonal control of the UGT1 locus was further verified in pregnant and nursing Tg-UGT1 mice. In maternal 14-day post-conception Tg-UGT1 mice, liver UGT1A1, UGT1A4, and UGT1A6 were induced, with the levels returning to near normal by birth. However, maternal liver UGT1A4 and UGT1A6 were dramatically elevated and maintained after birth, indicating that these proteins may play a critical role in maternal metabolism during lactation. With expression of the UGT1 locus confirmed in a variety of mouse tissues, these results suggested that the Tg-UGT1 mice will be a useful model to examine the regulatory and functional properties of human glucuronidation.

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Cited by 501 publications

References 105 publications

Receptor Activator of NF-κB Ligand Induction via Jak2 and Stat5a in Mammary Epithelial Cells

Receptor Activator of NF-κB Ligand Induction via Jak2 and Stat5a in Mammary Epithelial Cells

Trajectory Clustering: A Non-Parametric Method for Grouping Gene Expression Time Courses, With Applications to Mammary Development

Tissue-specific, Inducible, and Hormonal Control of the Human UDP-Glucuronosyltransferase-1 (UGT1) Locus

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