8-Prenylkaempferol accelerates osteoblast maturation through bone morphogenetic protein-2/p38 pathway to activate Runx2 transcription

Chiou, Wen-Fei; Lee, Chia-Hsin; Liao, Junyi; Chen, Chien-Chih

doi:10.1016/j.lfs.2010.12.009

Cited by 22 publications

(21 citation statements)

References 29 publications

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“…Khedgikar et al reported that kaempferol could facilitate cytoskeletal mineralization in osteoblasts by targeting keratin 14 (Krt-14) [8]. Chiou et al indicated that kaempferol could promote osteoblast maturation through bone morphogenetic protein-2 (BMP-2)/p38 pathway to activate runt-related transcription factor 2 (Runx2) [9]. Guo et al indicated that kaempferol could promote osteoblast differentiation via estrogen receptor signaling [10].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Kaempferol promotes proliferation, migration and differentiation of MC3T3-E1 cells via up-regulation of microRNA-101

Wang

Chen

Zhang

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Kaempferol promotes proliferation, migration and differentiation of MC3T3-E1 cells via up-regulation of microRNA-101

Wang

Chen

Zhang

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…B). Similarly other studies have also presented evidence that icariin and 8‐prenylkaempferol, the two flavonoids, promote osteoblast differentiation and mineralization through the BMP‐2/Runx2 pathway (Cao, et al ., ; Chiou et al ., ). Runx2 is critically regulates many of the genes that control the development of osteoblast differentiation such as ALP, Coll‐I, OCN, OPN, and bone sialoprotein (Lo et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Stimulative Effect of Ginsenosides Rg5:Rk1 on Murine Osteoblastic MC3T3‐E1 Cells

Ṣiddiqi

Ahn

Kang

et al. 2014

Phytotherapy Research

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Panax ginseng C.A. Meyer (P. ginseng), hereafter referred to as P. ginseng, is known to exert a wide range of pharmacological effects both in vitro and in vivo; however, few studies have investigated the effects of ginseng on bone metabolism. We therefore investigated the potential antiosteoporotic properties of ginseng on the growth and differentiation of murine MC3T3-E1 cells. Rg5:Rk1 is a mixture of protopanaxadiol-type ginsenosides, isolated from fresh P. ginseng root, via a repetitive steaming and drying process. In this study, we examined the stimulatory effects of Rg5:Rk1 on the differentiation and mineralization of MC3T3-E1 cells. Undifferentiated cells were treated with a range of concentrations of Rg5:Rk1 (1-50 µg/mL), and cell viability was measured with the 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Treatment with Rg5:Rk1 significantly increased cell viability in a dose-dependent manner. To investigate the possible mechanisms by which Rg5:Rk1 affects the early differentiation phase of MC3T3-E1 cells, the cells were treated with Rg5:Rk1 for 14-24 days before assessing the levels of multiple osteoblastic markers. The markers examined included alkaline phosphatase (ALP) activity type I collagen content (Coll-I), calcium deposition (by Alizarin Red S staining), extracellular mRNA expression of bone morphogenetic protein-2 (BMP-2), and the level of Runt-related transcription factor 2 (Runx2). Rg5:Rk1 treatment also increased the activities of proteins associated with osteoblast growth and differentiation in a dose-dependent manner. Overall, we found that the Rg5:Rk1 mixture of ginsenosides improved the osteoblastic function of MC3T3-E1 cells by increasing their proliferative capacity. This improvement is due to the action of Rg5:Rk1 on BMP-2, which is mediated by Runx2-dependent pathways.

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“…19 Recently, its alternative role has been emphasized in carcinogenesis. 20 In this study, we found there was focal and weak expression of Runx2 in normal thyroid and benign thyroid lesions, and diffuse and intense expression in thyroid carcinomas including PCs, FCs and UCs.…”

Section: Discussionmentioning

confidence: 99%

Transcription factor Runx2 is a regulator of epithelial–mesenchymal transition and invasion in thyroid carcinomas

Niu

Kondo

Nakazawa

et al. 2012

Laboratory Investigation

103

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Runx2/Cbfa1 is a member of the Runt-related transcription factor family and is an essential regulator of osteoblast/ chondrocyte differentiation. Recently, aberrant expression of Runx2 and its oncogenic functions have been identified in the progression and metastasis of human cancers. In this study, we investigated the expression profile of Runx family genes in normal thyroid tissue, non-neoplastic but abnormal thyroid tissue, various types of thyroid tumors and representative human thyroid carcinoma cell lines. Using reverse transcriptase-PCR and western blotting, we found that Runx2 was consistently upregulated in papillary carcinomas (PCs) and thyroid carcinoma cell lines compared with normal thyroid tissue. With immunohistochemistry, we observed negative or focal immunoreactivity of Runx2 in the nuclei of normal thyroid follicular cells. None of the non-neoplastic thyroid tissues, including Graves' thyroid and adenomatous goiter, had diffuse positivity of Runx2. Expression of Runx2 in benign follicular adenomas varied from negative to diffusely positive. Meanwhile, all malignant thyroid tumors showed some Runx2 immunopositivity. It was diffuse and intense in 83% (19/23) of PCs, 71% (5/7) of follicular carcinomas (FCs) and 40% (4/10) of undifferentiated carcinomas (UCs). In thyroid carcinoma cell lines, the MEK inhibitor U0126 suppressed Runx2, suggesting an association of the MAPK/ERK pathway with Runx2 regulation. Effective silencing of Runx2 by short interfering RNA (siRNA) demonstrated downregulation of EMT-related molecules (SNAI2, SNAI3 and TWIST1), MMP2 and vasculogenic factors (VEGFA and VEGFC) in thyroid carcinoma cells. We also confirmed that Runx2 silencing suppresses thyroid carcinoma cell invasion in transwell assays. In conclusion, this study provides insight into the potential molecular mechanism of thyroid cancer invasion. Our data suggest that enhanced Runx2 is functionally linked to tumor invasion and metastasis of thyroid carcinoma by regulating EMT-related molecules, matrix metalloproteinases and angiogenic/lymphangiogenic factors.

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8-Prenylkaempferol accelerates osteoblast maturation through bone morphogenetic protein-2/p38 pathway to activate Runx2 transcription

Cited by 22 publications

References 29 publications

RETRACTED ARTICLE: Kaempferol promotes proliferation, migration and differentiation of MC3T3-E1 cells via up-regulation of microRNA-101

RETRACTED ARTICLE: Kaempferol promotes proliferation, migration and differentiation of MC3T3-E1 cells via up-regulation of microRNA-101

Stimulative Effect of Ginsenosides Rg5:Rk1 on Murine Osteoblastic MC3T3‐E1 Cells

Transcription factor Runx2 is a regulator of epithelial–mesenchymal transition and invasion in thyroid carcinomas

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