8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures

Abstract

Oxidative DNA damage is considered to play an important role in pathophysiological processes, ageing and cancer. So far major interest has been on measuring 8-hydroxy-2'-deoxyguanosine (8-OHdG), the preferred methods relying on HPLC or GC-mass spectrometry. The high biological relevance of 8-OHdG is due to its ability to induce G-->T transversions, which are among the most frequent somatic mutations found in human cancers. Effects of workplace exposures on the level of white blood cell 8-OHdG or urinary 8-OHdG have been reported with controversial results. Exposures examined include asbestos, azo-dyes, benzene, fine particulate matter (PM(2.5)), glassworks, polycyclic aromatic hydrocarbons (PAHs), rubber manufacturing, silica, metals, styrene, toluene and xylenes. The available data indicate that there is still a lack of well established dose-response relations between occupational or environmental exposures and the induction of 8-OHdG. Smoking has been most consistently identified as a confounder for 8-OHdG, but various occupational studies did not reveal higher levels of 8-OHdG in smokers. Despite the conflicting results, the reported studies show promise for 8-OHdG as a biomarker of oxidative stress associated with chemical exposure. However, there are critical aspects related to the analytical challenge, artifactual production of 8-OHdG, inter- and intra-individual variation, confounding factors and inter-laboratory differences, implying that further work is needed to reach a consensus on the background level of 8-OHdG.

Assessment of 8-oxo-7,8-dihydro-2′-deoxyguanosine as a marker of oxidative DNA damage in gasoline filling station attendants

“…and non-smokers. Smoking has been consistently identified as a confounder for 8-oxodG, but various occupational studies did not reveal higher levels of 8-oxodG [24] in smokers as indicated by the present study. A study by Besarati Nia et al [25] reported that the level of 8-oxodG was consistently increased among smokers.…”

Low 8-oxo-7,8-dihydro-2′-deoxyguanosine levels and influence of genetic background in an Andean population exposed to high levels of arsenic

“…Although it is clear that several environmental exposures result in increased levels of 8-OxodG [49], the relation between levels of urinary 8-OxodG and future risk of health effects, such as cancer, is not well characterized. There is one Danish prospective study on 8-OxodG and lung cancer showing that increasing levels of 8oxodG…”

Study of Oxidants and Antioxidants in Addicts

“…The 8-OxodG is the most frequent mutation in human cancers (33). Cooke et al (34) reported elevated concentrations of 8-oxodG in a high quantity of cases of several pre-cancerous and cancerous conditions.…”