72-Hour perfusion of the isolated spleen at normothermia

Pabst, Reinhard; Trepel, F.

doi:10.1007/bf01851943

Cited by 13 publications

(3 citation statements)

References 10 publications

(10 reference statements)

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“…The spleen is one of few tissues on which quantitative information on the fuels of respiration is lacking. There are some data on oxygen and glucose utilization and lactate production in pig spleen perfused with haemoglobin-free perfusates (Pabst & Trepel, 1974). However, these perfusions are likely to be oxygen deficient and this is reflected in a much lower oxygen consumption and higher lactate production than obtained in our studies on rat spleen.…”

Section: Discussionmentioning

confidence: 47%

“…The perfused spleen has been utilized for investigations in the rat (Ford & Gowans, 1967;Ford, 1969), pig and human (Pabst & Trepel, 1974), where it has been used for the study oflymphocyte migration. However these studies employed heparinized, low haematocrit or erythrocytefree perfusates and revealed little information on spleen metabolism.…”

Section: Introductionmentioning

confidence: 99%

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The fuel of the spleen. Studies using a new method for perfusing the rat spleen with whole blood

Mindham

Mayes

1989

Biochemical Journal

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1. An improved rat spleen perfusion is described incorporating a method of defibrination which avoids the use of heparin and enables the spleen to be perfused with rat blood for several hours at a haematocrit of 40% and for 12 h or more at a haematocrit of 20%. 2. Glucose oxidation accounted for 11.6% of the total oxygen consumption but this represented only 8% of total glucose uptake, which was largely converted to lactate and released into the perfusate. However, significant amounts of lactate were oxidized. These results can be explained by the presence of at least two cell populations, one emphasizing the anaerobic oxidation of glucose and the other aerobic metabolism, particularly of lactate. 3. Non-esterified fatty acid and 3-hydroxybutyrate, when available at physiological concentrations, were shown to be major oxidative fuels of the spleen. 4. Chylomicron triacylglycerol was hydrolysed readily and taken up, but not oxidized extensively.

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Section: Discussionmentioning

confidence: 47%

Section: Introductionmentioning

confidence: 99%

The fuel of the spleen. Studies using a new method for perfusing the rat spleen with whole blood

Mindham

Mayes

1989

Biochemical Journal

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“…The perfusion system, as shown diagrammatically in Figure 1, is comparable to the system previously described (Pabst & Trepel 1974, Reinecke & Pabst 1980 and, therefore, only a brief outline is given. The system consisted of a roller pump, a perfusion chamber, an oxygenator and a regulating system to keep the pH constant.…”

Section: Perfusion Systemmentioning

confidence: 99%

Proliferation of Human Lymphoid Cells Lymphocytopoiesis and Cell Cycle Parameters in Isolated Perfused Human Spleens

Pabst

Reinecke

1981

Scandinavian Journal of Haematology

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Parameters of the cell cycle of lymphoid cells and the rate of production of small lymphocytes and mature plasma cells were estimated in human spleens. 12 normal and 1 enlarged spleen with lymphatic hyperplasia were labelled either by a pulse or continuously with 3H-TdRthymidine during normothermic perfusion of the isolated spleens. Biopsies of splenic tissue were taken at different intervals and evaluated autoradiographically. The mean initial labelling index of immunoblasts was 73 % and for plasmoblasts 52 %. The labelling index for small lymphocytes and mature plasma cells increased initially from 0 % to 1 %, and up to 25 % respectively after 14 h. The duration of the S-phase, as determined by double labelling, was 8.26 h for lymphoid blasts. Data from the percentage labelled mitosis curve resulted in a minimal tG2 of about 1 h and tG2 + tM of about 4 h. The estimated generation time was about 11.5 h. The results found on the hyperplastic spleen were comparable to those on the normal spleen.

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Comparison of in vitro and in vivo cell cycle parameters of lymphoid cells in Pig spleens

Pabst

Trepel

1976

Cell Tissue Res.

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Parameters of the cell cycle of lymphoid cells were estimated by analyzing percent labeled mitoses curves after a 3H-thymidine flash. Either anaesthetized pigs were labeled and multiple biopsies taken from the spleen in vivo or isolated perfused pig spleens were labeled in vitro. The data from in vivo and in vitro experiments were very similar. The mean values for cell cycle parameters were: 20.2 to 20.5 hours for the generation time, about 0.5 to 1 hour for G2, about 1.2 to 1.3 hours for M; about 17 to 16.4 hours for S and about 1.5 to 1.7 hours for G1. The mean grain count halving time of labeled mitoses was in accordance with the measured generation time. The isolated perfused spleen seems to give results equal to in vivo data and could, therefore, be employed as a model for studying cell cycle parameters not only in animal but also in human lymphoid tissue.

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72-Hour perfusion of the isolated spleen at normothermia

Cited by 13 publications

References 10 publications

The fuel of the spleen. Studies using a new method for perfusing the rat spleen with whole blood

The fuel of the spleen. Studies using a new method for perfusing the rat spleen with whole blood

Proliferation of Human Lymphoid Cells Lymphocytopoiesis and Cell Cycle Parameters in Isolated Perfused Human Spleens

Comparison of in vitro and in vivo cell cycle parameters of lymphoid cells in Pig spleens

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