70% Ethyl Alcohol as Skin Cleanser for Fungus Cultures

Loewenthal, Kurt

doi:10.1001/archderm.1964.01590290091013

Cited by 2 publications

(4 citation statements)

References 9 publications

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“…The literature reveals several comparative evaluations regarding the efficiency of direct KOH mount and culture (2,4,5,7,8,11,12). Everett (2) stated that 90% of the cases of tinea pedis may be revealed by performing one microscopic examination, and two such preparations would discover 98%.…”

Section: Discussionmentioning

confidence: 99%

“…Everett (2) stated that 90% of the cases of tinea pedis may be revealed by performing one microscopic examination, and two such preparations would discover 98%. Strauss and Kligman (8) reported that a K O H mount is superior to culture, while Loenenthal (5) found that only 33% of the K O H positive samples were positive in culture. Our study reveals a correlation of 45.4% between KOH mount and culture.…”

Section: Discussionmentioning

confidence: 99%

“…Of the 220 samples, 97.3% were positive in the direct smear and only 48.2% in culture. Of the 106 samples positive in culture, six (5.7%) were negative in the direct preparation, and of the 214 K O H positive samples, 108 (50.5%) were negative by culture including 4 samples with a very Discrepancies between direct KOH m o u n t a n d culture have been observed in o t h e r laboratories too (3,5,12). This can b e explained b y : presence of dead particles in samples f r o m treated patients; similarity between h y p h a e of saprophytic fungi (Alternaria, Aspergillus, Penicilliuin etc.)…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Direct Microscopic Examination Versus Culture in the Diagnosis of Superficial Fungal Infections

Lefler

Haim

Merzbach

2009

Mycoses

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Summary: Five hundred eighty six samples of skin and nail scrapings or hair clinically suspected of superficial fungal infection were studied by direct smears and cultures. 366 (62.5%) of these samples were negative by both methods. Of the remaining 220 (37.5%) samples, 114 (51.8%) were positive by the direct KOH preparation, 6 (2.7%) by culture and 100 (45.5%) by both methods. Most (72.7%) of the 214 (97.3%) KOH positive smears showed a low to high hyphal content, and 4 (1.9%) of them were culturally negative although they possessed a very high amount of hyphae. Discrepancies between the direct microscopic examination and the subsequent culture are well known in the literature and therefore this study strongly suggests the necessity of both tests for the diagnosis of superficial mycoses. Zusammenfassung: 586 Proben von Hautschuppen, Nagelmaterial und Haaren, bei denen klinisch an eine oberflächliche Pilzinfektion gedacht worden war, wurden im Nativpräparat und mit der Pilzkultur untersucht. 366 (62,5%) von diesen Proben waren in beiden Untersuchungsgängen negativ. Von den restlichen 220 (37,5%) Proben waren 114 (51,8%) nur im Nativpräparat (KOH) positiv, 6 (2,7%) zeigten nur ein positives Kulturergebnis, während 100 Proben (45,5%) bei beiden Methoden einen positiven Befund ergaben. Von den 214 (97,3%) positiven Nativpräparaten zeigten die meisten (72,7%) einen niedrigen bis hohen Gehalt an Pilzhyphen und 4 (1,9%) von ihnen waren in der Kultur negativ, obwohl sie einen sehr hohen Gehalt an Hyphen aufwiesen. Diskrepanzen zwischen den Ergebnissen des Nativpräparates und denen der Pilzkultur sind in der Literatur gut bekannt. Daher stellt auch diese Untersuchung einen starken Hinweis darauf dar, daß beide Untersuchungsmethoden für die Diagnosestellung von oberfflächlichen Mykosen benötigt werden.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of Direct Microscopic Examination Versus Culture in the Diagnosis of Superficial Fungal Infections

Lefler

Haim

Merzbach

2009

Mycoses

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“…It is important to consider some non‐dermatophytic filamentous fungi from nail samples as possible aetiologic agents of onychomycosis [5]. It is thus useful to culture all nail samples on Sabouraud medium without cycloheximide (Sabouraud) to allow the isolation of moulds and yeasts as well as on cycloheximide containing Sabouraud medium (Sabouraud+C) allowing the growth of dermatophytes [6].…”

Section: Discussionmentioning

confidence: 99%

The influence of alcohol disinfection of nail samples in the laboratory

Willemsen

Breynaert

Lauwers

1999

Mycoses

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Samples of two hundred finger- and toenails cultured after a 70% alcohol disinfection for 2 min were compared with non-disinfected samples cultured on a classical way. Alcohol treatment caused a significant decrease in isolation of yeast and moulds. Both organisms were cultivated at 25.5 and 31%, respectively, when not treated with alcohol, whereas the isolation rate dropped to 10 and 17.5%, respectively, after alcohol treatment. Isolation of dermatophytes decreased from 27.5 to 23.5% when alcohol disinfection was used. In conclusion, although alcohol disinfection of nail samples in the laboratory effectively decreases the isolation rate of unwanted contaminants on Sabouraud glucose agar without cycloheximide, it hampers the isolation of dermatophytes and pathogenic yeasts on cycloheximide-containing media.

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70% Ethyl Alcohol as Skin Cleanser for Fungus Cultures

Cited by 2 publications

References 9 publications

Evaluation of Direct Microscopic Examination Versus Culture in the Diagnosis of Superficial Fungal Infections

Evaluation of Direct Microscopic Examination Versus Culture in the Diagnosis of Superficial Fungal Infections

The influence of alcohol disinfection of nail samples in the laboratory

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