[7] Surgical procedures for intravascular delivery of plasmid DNA to organs

Budker, Vladimir G.; Williams, Phillip; Hanson, Kristine; Wolff, Jon A.

doi:10.1016/s0076-6879(02)46052-1

Cited by 17 publications

(7 citation statements)

References 12 publications

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“…The technique used in rats by Budker et al [3,23] was adapted to non-human primates (rhesus macaques [24]) and to mice and dogs [19]. Another technique, which involves clamping of the abdominal aorta and vena cava and subsequent injection by the tail vein or artery, also allowed good transduction of a whole limb [25].…”

Section: Discussionmentioning

confidence: 99%

Femoral intra‐arterial injection: a tool to deliver and assess recombinant AAV constructs in rodents whole hind limb

Gonin

Arandel

Wittenberghe

et al. 2005

The Journal of Gene Medicine

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With the aim of simplifying recombinant-adeno-associated virus (rAAV) delivery in muscle, a new femoral intra-arterial technique was designed and tested in rodents (rats and mice). Two serotypes, several promoters and transgenes (reporter or therapeutic) were tested using this administration route. The new route is both easy to perform and efficient. Its usefulness as a tool to assess gene delivery constructs in the muscle was established in the context of recombinant AAV serotypes 1 and 2, and with the ubiquitous CMV and two muscle-specific (C5-12 and CK6) promoters. Both serum monitoring of a secreted protein (murine alkaline phosphatase: muSEAP) and slide staining were used to compare the different constructs. Significantly different patterns of expression in kinetics of expression (muSEAP) and homogeneity of fiber transduction (staining) were evidenced with the different promoters tested, and compared with intra-muscular expression patterns. Detailed studies of differential transduction in leg and thigh muscles showed equivalent efficacy, except in rectus femoris, and to a lesser extent in soleus. In light of these results and prior data, intra-arterially mediated gene transfer mechanism is discussed.

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Section: Discussionmentioning

confidence: 99%

Femoral intra‐arterial injection: a tool to deliver and assess recombinant AAV constructs in rodents whole hind limb

Gonin

Arandel

Wittenberghe

et al. 2005

The Journal of Gene Medicine

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“…The principle of the hydrodynamics‐based procedure could be applicable to an organ‐restricted gene delivery method; i.e. targeting to the organs such as the liver, the kidney and the hindlimb muscles by injection via a suitable vein or artery with transient occlusion of the outflow as demonstrated previously 18–23. In actual fact, Eastman et al 24 recently reported the catheter‐mediated hydrodynamics‐based delivery of pDNA using isolated rabbit liver.…”

Section: Introductionmentioning

confidence: 89%

Hydrodynamics‐based procedure involves transient hyperpermeability in the hepatic cellular membrane: implication of a nonspecific process in efficient intracellular gene delivery

Kobayashi

Nishikawa

Hirata

et al. 2004

The Journal of Gene Medicine

113

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“…Vascular delivery could be systemic or regional in which injections are into specific vessels that supply a target tissue. 7 The intravascular delivery of adenoviruses or cationic lipid-DNA complexes in adult animals mostly results in expression in vascular-accessible cells such as endothelial cells or hepatocytes reached via the sinusoid fenestrae. Efficient transgene expression in hepatocytes throughout the liver can be obtained following delivery of naked pDNA via the portal vein, the hepatic vein, or the bile duct in mice and rats.…”

Section: Prospectsmentioning

confidence: 99%