[7] Paper chromatography of antibiotics

Betina, V.

doi:10.1016/0076-6879(75)43083-x

Cited by 11 publications

(2 citation statements)

References 133 publications

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“…Anti-gastric carcinoma VEGF MCAB and 5-FUAb-NPs, the immune conjugate,combined with the 5-FU nanoparticles, only had a specific targeted orientating function on the gastric carcinoma cells, suggesting that anti-gastric carcinoma VEGF MCAB can play a role in tissue targeting. The 5-FU is the choice drug for treating malignant gastrointestinal tumors, being a time-dependent drug that needs a delayed time to exert its optimal anti-cancer effect [6,7] . The findings of our study showed that following intravenous administration, the concentration of 5-FU-AbNPs in the tumor increased 2 or more times, compared to that of the peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

Studies on the distribution and radioimmunoimaging of 99mTc-labeled 5-fluorouracil loaded immunological nanoparticles in tissues and human gastric carcinoma xenografts

Huang

Liu

Zhu

et al. 2007

Chin. J. Clin. Oncol.

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OBJECTIVE To explore the method of preparation of 99m Tc labeled Anti-VEGF McAb 5-FU loaded polylactic acid nanoparticles ( 99m Tc-5-FU-Ab-NPs), and investigate the biological distribution of the nanoparticles in human gastric carcinoma xenografts. METHODS Anti-VEGF monoclonal antibodyes (MCAB) in 5-FU-Ab-NPs were labeled with 99m Tc using a modified Schwarz method. After isolation of the 99m Tc-5-FU-Ab-NPs using a Sephadex G-250 column, the labeling percentage and radiochemical purity were determined using paper chromatography. The immunocompetence of the 99m Tc-5-FU-Ab-NPs as tumor markers was determined using ELISA and immunohistochemistry. 99m Tc-5-FU-Ab-NPs (experimental group), 99m Tc-labelled murine multiclonal IgG loaded polylactic acid and nanoparticles (control group) were injected via the tail vein into SCID mice bearing human gastric carcinoma. A radio-immunity ECT image was developed at 2 and 6 h after the injection. Following the ECT imaging, the mice were sacrificed, their tissue and tumor radioactivity distribution determined, and percentage of the injected-dose per gram (%ID/g) and tumor/ nontumor (T/NT) ratio calculated. High performance liquid chromatography (HPLC) was used to determine the 5-FU concentration in the tumor tissue and blood in the mice of both groups. RESULTSThe percentage of 99m Tc-5-FU-Ab-NPs labeling was 90%~95%. There was no obvious decrease in the antibody activity before and after labeling. The radio-immuno-imaging (RII) showed that the tumor image had developed 2 h after injection of the 99m Tc-5-FU-Ab-NPs, and with time it was clearer at the 6th hour following the injection. The %ID/g of the tumor tissue at both 2 h and 6 h after the injection was significantly higher compared to the control group. The tumor %ID/g and the tumor to blood activity ratio (TB) of the experimental group at 6 h following the injection increased compared to that at 2 h, and at the same time, 5-FU concentration in the tumor of the experimental group continuously increased over time, and showed a significant difference compared to the 5-FU concentration in the tumor of the control group. CONCLUSION The 99m Tc-5-FU-Ab-NPs prepared in this study are adequate to meet the demands of the RII, and the immune targeting ability of the anti-VEGF MCAB is reliable. Six hours after injection, the 99m Tc-5-FU-Ab-NPs showed a relatively high specific concentration shadow in the human gastric carcinoma xenografts.

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Section: Discussionmentioning

confidence: 99%

Studies on the distribution and radioimmunoimaging of 99mTc-labeled 5-fluorouracil loaded immunological nanoparticles in tissues and human gastric carcinoma xenografts

Huang

Liu

Zhu

et al. 2007

Chin. J. Clin. Oncol.

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“…The following steroid separations were carried out: a-and (3-ecdysone on Quantum preadsorbent silica gel, chloroform-methanol (4:1) solvent, UV detection (323B); 6 major androgens and estrogens simultaneously on preadsorbent silica gel layers developed with chloroform-ethyl acetate (80:20) at 32-72% humidity (324B); thromboxanes from prostaglandins by one-and two-dimensional TLC in 9 different solvent systems (23B); 17-ketosteroid 2,4-dinitrophenyl hydrozones on silica gel using the solvent dichloromethane-ethyl acetate (186:14) (31B)\ estradiol, testosterone, and 5a-dihydrotestosterone in the same plasma sample by overflow PC development (65B); isomers and epimers of the pregnane series by multiple development on silica gel or neutral or basic alumina (ll4B)\ 8 corticosteroids from their respective 17-oxo oxidation products on silica gel using dichloromethanedioxane-water (100:50:50 and 120:30:50), UV detection (237B); 11 androgens with benzene-ethyl acetate (2:1) and benzene-acetone (7:1) (2D) and 5 estrogens with benzene-acetone (8:1) on silica gel (291B); corticosteroids on polyamide layers with dichloromethane-methanol (98:2) solvent (recommended as a substitute for LH-20 column chromatography) (329B); steroids extracted from urine by 2D-PC (double development in both direction) with dichloroethane-ethanol (92.5:7.5) and diisopropyl ether-ethanol (95:5), detection with tetrazolium reagent (346B); steroidal ketones, oximes, and lactams on silica gel, ceric sulfate or iodine detection (343B); 10 steroidal lactams, 19 steroidal tetrazoles, 17 basic azasteroids, and 9 quaternary azasteroids on silica gel (344B); prostaglandins E2 and F2a from their pulmonary metabolites on silica gel plates impregnated with both silver nitrate and boric acid (345B); and steryl acetates on silver nitrate-impregnated silica gel, petroleum ether-diethyl ether (99:1) solvent, ceric ammonium nitrate detection reagent (215B). Various chromatographic techniques were compared for determination of urinary neutral steroid profiles (147B).…”

Section: Applicationsmentioning

confidence: 99%