7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff’s reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section

Takase, Hiroshi; Murase, Takayuki; Hachisuka, Daisuke; Sakamoto, Yuma; Sugiura, Masahiro; Nakano, Satsuki; Fujii, Keiichiro; Masaki, Ayako; Inagaki, Hiroshi

doi:10.1080/10520295.2022.2101144

Cited by 2 publications

(7 citation statements)

References 11 publications

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“…In a recent histologic study, we showed that the distribution of AMC staining as observed under UV excitation was identical to PAS staining observed under bright field illumination. 7 In the present cytologic study, we found that AMC signal distribution was identical to that obtained by PAS staining and that AMC stain- PAS is an ancillary stain that is particularly useful for visualizing mucus-or glycogen-rich tumor cells, 1 and Pap/AMC and Giemsa/ AMP stains are expected to contribute to a more accurate cytopathologic diagnosis of various tumors as illustrated in this study in several cytologic instances including mucin-producing endometrial carcinoma, adenoid cystic carcinoma, and metastatic signet ring cell carcinoma. 9 On the other hand, candidiasis and trichomoniasis are easily overlooked in cytologic specimens treated with standard stains.…”

Section: Discussionmentioning

confidence: 99%

“…As described in our previous study, using an AMC working solution that had been stored for 1 month caused the fluorescence of the AMC to decrease by one third compared with when it was freshly prepared. 7…”

Section: Discussionmentioning

confidence: 99%

“…To prepare the AMC working solution, 25 μL AMC stock solution and 2.5 mg 2‐picoline borane, a nontoxic reducing reagent, were dissolved in 50 mL aqueous citric acid solution (1 mg/mL citric acid monohydrate). The stability of AMC stock and working solutions were described in our previous study 7 . AMC staining was performed in the dark field at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The stability of AMC stock and working solutions were described in our previous study. 7 AMC staining was performed in the dark field at room temperature. Details of the AMC staining procedures are shown in Figures 1 and 2 for ethanol-fixed and air-dried specimens, respectively.…”

Section: Amc Stock Solution and Amc Working Solutionmentioning

confidence: 99%

“…Aqueous 7‐amino‐4‐methylcoumarin (AMC; CID: 92249) is colorless under bright field but exhibits strong fluorescence under ultraviolet (UV) light. We found that AMC can be used as a Schiff reagent to visualize aldehydes and we recently reported that using H&E combined with AMC, H&E, and PAS‐like histologic images can be obtained under bright and UV fields, respectively, employing a single tissue section 7 . In this study, we applied AMC staining to cytology, and evaluated the usefulness of combining Pap and AMC (Pap/AMC) and Giemsa and AMC (Giemsa/AMC) in cytopathologic diagnosis.…”

Section: Introductionmentioning

confidence: 99%

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Fluorescent periodic acid Schiff‐like staining combined with standard cytologic staining of the same cytologic specimen may facilitate cytopathologic diagnosis

Hachisuka,

Nakano,

Takase

et al. 2023

Diagnostic Cytopathology

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BackgroundPeriodic acid Schiff (PAS) staining which detects glycogen and mucosubstances is frequently used as an ancillary method for an accurate cytopathologic diagnosis. Unfortunately, cytologic slides for PAS stain are not routinely prepared. Aqueous 7‐amino‐4‐methylcoumarin (AMC) is colorless and transparent under bright field illumination but exhibits strong fluorescence under ultraviolet (UV) light and can be used as a Schiff reagent. We recently reported that combining [author: Please define (H&E) in the first occurrence if necessary.]H&E and AMC is useful for histopathologic diagnosis of various disease conditions. In this study, we investigated whether standard cytologic staining (Papanicolaou [Pap] and Giemsa) combined with AMC was useful for cytopathologic analysis.MethodsSpecimens of non‐neoplastic human tissues and archived cytologic specimens of various disease conditions were stained with a combination of Pap and AMC (Pap/AMC) or Giemsa and AMC (Giemsa/AMC).ResultsThe addition of AMC had no significant effect on Pap or Giemsa staining, and the cytomorphology under bright field microscopy was perfectly preserved. The AMC fluorescent signals observed under UV light were intense and the staining pattern was identical to that obtained by PAS staining. Diastase digestion differentiated glycogen from other AMC‐positive elements. The efficacy of using Pap/AMC and Giemsa/AMC for archived cytologic specimens was demonstrated in several diseases including cases of endometrial carcinoma, adenoid cystic carcinoma, metastatic signet‐ring cell carcinoma, candidiasis, and trichomoniasis.ConclusionPap/AMC and Giemsa/AMC are useful in aiding cytopathologic diagnosis especially when the information gained from PAS staining is critical and cytologic specimens for PAS are not available.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Amc Stock Solution and Amc Working Solutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fluorescent periodic acid Schiff‐like staining combined with standard cytologic staining of the same cytologic specimen may facilitate cytopathologic diagnosis

Hachisuka,

Nakano,

Takase

et al. 2023

Diagnostic Cytopathology

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A Simple and Effective Protocol for the Pechmann Reaction to Obtain 4-Methylcoumarin Derivatives Using a High-Speed Mixer Ball Mill Process

Becerra-Anaya,

Merchán Arenas,

Kouznetsov

2023

Chemistry

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We hereby report a simple and efficient method for the preparation of 4-methylcoumarins series, including Coumarin 120 (7-amino-4-methylcoumarin) from phenols (or naphthols) and ethyl acetoacetate in the presence of 3 mol% InCl3. Coumarins were obtained in good yields (52–92%) through Pechmann condensation, under a rapid and environmentally friendly protocol using a high-speed ball mill mixer at room temperature, with short reaction times, under solvent-free conditions.

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7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff’s reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section

Cited by 2 publications

References 11 publications

Fluorescent periodic acid Schiff‐like staining combined with standard cytologic staining of the same cytologic specimen may facilitate cytopathologic diagnosis

Fluorescent periodic acid Schiff‐like staining combined with standard cytologic staining of the same cytologic specimen may facilitate cytopathologic diagnosis

A Simple and Effective Protocol for the Pechmann Reaction to Obtain 4-Methylcoumarin Derivatives Using a High-Speed Mixer Ball Mill Process

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