7

Abstract

Learning Objectives: Reactive oxygen species (ROS) is known to contribute to the development of organ dysfunction during sepsis. Enteral feeding in the critically ill patients is crucial and immune-modulating diet (IMD) might have potential to prevent oxidative stress and inflammation. However we are not sure whether IMD containing whey protein as a complete peptide and EPA is suppressed excessive ROS production during sepsis. The present study was conducted to investigate the ROS production by the effect of IMD containing whey protein and EPA in a rodent sepsis model. Methods: Sprague-Dawley rats were fed with IMD containing whey protein and EPA or control diet (CD) for 7 days. Rats were injected with lipopolysaccharide (LPS) 10mg/kg or normal saline (NS) via intraperitoneally. Blood plasma samples were collected at 5 hours after injection for measurement of derivatives of reactive oxygen metabolites (d-ROMs) as an oxidative stress marker. Biological anti-oxidant potential (BAP) and oxy adsorbent test (OXY) as total anti-oxidant. BAP/dROMs and OXY/dROMs were used for compare between groups. Results: In normal S-D rat BAP/d-ROMs levels were (10.5 ± 0.3) and OXY/d-ROMs levels were (1.3 ± 0.03). In septic rat treated by LPS 10mg/kg BAP/d-ROMs and OXY/d-ROMs levels were (11.4 ± 0.9) and (1.1 ± 0.06) respectively. OXY/d-ROMs levels were significantly decreased between normal and septic rats. BAP/d-ROMs levels were lower in IMD-LPS (15.01 ± 1.0) group than CD-LPS (12.6 ± 0.4), IMD-NS (12.4 ± 0.8), CD-NS (12.5 ± 1.2) groups. OXY/d-ROMs levels were significantly higher in IMD-LPS (1.74 ± 0.1) than CD-LPS (1.32 ± 0.1), IMD-NS (1.5 ± 0.1), CD-NS (1.5 ± 0.1). Conclusions: These results indicate IMD suppressed excessive ROS production and enhanced total antioxidant in a rodent sepsis model. IMD with whey protein and EPA has potential to prevent oxidant injury.