7,8-Dihydroxyflavone Protects High Glucose-Damaged Neuronal Cells against Oxidative Stress

Abstract

Oxidative stress is considered a major contributor in the pathogenesis of diabetic neuropathy and in diabetes complications, such as nephropathy and cardiovascular diseases. Diabetic neuropathy, which is the most frequent complications of diabetes, affect sensory, motor, and autonomic nerves. This study aimed to investigate whether 7,8-dihydroxyflavone (7,8-DHF) protects SH-SY5Y neuronal cells against high glucose-induced toxicity. In the current study, we found that diabetic patients exhibited higher lipid peroxidation caused by oxidative stress than healthy subjects. 7,8-DHF exhibits superoxide anion and hydroxyl radical scavenging activities. High glucose-induced toxicity severely damaged SH-SY5Y neuronal cells, causing mitochondrial depolarization; however, 7,8-DHF recovered mitochondrial polarization. Furthermore, 7,8-DHF effectively modulated the expression of pro-apoptotic protein (Bax) and anti-apoptotic protein (Bcl-2) under high glucose, thus inhibiting the activation of caspase signaling pathways. These results indicate that 7,8-DHF has antioxidant effects and protects cells from apoptotic cell death induced by high glucose. Thus, 7,8-DHF may be developed into a promising candidate for the treatment of diabetic neuropathy.

The TrkB agonist 7,8-dihydroxyflavone improves sensory-motor performance and reduces lipid peroxidation in old mice

“…Although the antioxidant enzyme activities, SOD and CAT, were not affected by drug administration, the lipid peroxidation was reduced. In a recent study made with neuronal cells, 7,8-DHF protected high glucose-damaged cell against oxidative stress (Cho et al 2019). Powerful antioxidant action of 7,8-DHF has been demonstrated in several studies against in scopolamine-induced oxidative stress (Chen et al 2014), glutamate-induced toxicity (Chen et al 2011), cerebral ischemia injury (Wang et al 2014).…”

Esculetin Prevents the Induction of Matrix Metalloproteinase-1 by Hydrogen Peroxide in Skin Keratinocytes

“…The primary extracellular signal-regulated kinase (ERK) 2 (sc-1647) and phospho-ERK1/2 (sc-7383) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The primary Actin (A2066) antibody was purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA) [17].…”

The Emerging Role of BDNF/TrkB Signaling in Cardiovascular Diseases

“…However, the authors did not provide direct causal evidence supporting BDNF/TrkB contributed to cardiac remodeling. Lately, another study found that 7,8-DHF protected neurons from high glucose-induced oxidative stress [ 53 ]. However, the potential role of 7,8-DHF in high glucose-induced cardiomyocyte injury and diabetic cardiomyopathy is undetermined.…”