7,8-DHF Treatment Induces Cyr61 Expression to Suppress Hypoxia Induced ER Stress in HK-2 Cells

Abstract

Acute kidney injury (AKI) is a common syndrome which is strongly linked to high morbidity and mortality. Hypoxia is the leading cause of AKI and the proximal renal tubular cells are the most damaged part in the kidney during this period. It has been observed that 7,8-dihydroxyflavone (7,8-DHF) plays a protective role by acting on antiapoptosis and antioxidative stress. In this study we explored functions of 7,8-DHF in protecting human proximal tubular cell line HK-2 from hypoxia insults. We observed that treatment of 7,8-DHF could improve the viability of ischemic cell. Mechanistically, we found that 7,8-DHF could elevate the expression of cysteine-rich protein 61 (Cyr61), a protective immediate early gene in AKI. In addition, treatment of 7,8-DHF decreased CCAAT/enhancer-binding protein homologous protein (CHOP) expression, which is a marker protein during endoplasmic reticulum (ER) stress activation. Intriguingly, overexpression of Cyr61 significantly reduced CHOP expression. Taken together, our results provide novel insights into the possible protective role of 7,8-DHF by activating Cyr61 signaling and suppressing ER stress in hypoxic HK-2 cells which have potential clinical implications for the treatment of AKI.

Identifying hub genes associated with clinical characteristicsin IgA nephropathy by Weighted Gene Co-expression Network Analysis

“…In tubulointerstitium, there were 480 DEGs between IgA nephropathy and healthy control. [43,44]. Therefore, our results of WGCNA and Gene ontology enrichment analysis were consistent with previous studies results, at least in part, Cyr61 as a matrix molecule related to apoptosis although the underlying mechanisms of proteinuria in IgA nephropathy still need to be explored.…”

An underutilized orphan tuber crop—Chinese yam : a review

“…Micropropagation has been used to generate Chinese yam microtubers in tissue culture, which are useful to avoid the carryover of viruses during long-term vegetative propagation (Nagasawa and Finer 1989 ; Kohmura et al 1995 ; Kadota and Niimi 2004 ; Li et al 2014 ). Reference genes suitable for quantitative expression analysis have also been identified to enable further molecular studies (Zhao et al 2016 ).…”

Molecular network, pathway, and functional analysis of time-dependent gene changes related to cathepsin G exposure in neonatal rat cardiomyocytes

“…The encoded protein interacts with several integrins and with heparan sulfate proteoglycan 23 . This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation 24, 25 . Previous study found that CYR61 had an anti-apoptotic effect on cardiac myocytes under oxidative stress and a proangiogenic property 26 .…”