6S-Like scr3559 RNA Affects Development and Antibiotic Production in Streptomyces coelicolor

Abstract

Regulatory RNAs control a number of physiological processes in bacterial cells. Here we report on a 6S-like RNA transcript (scr3559) that affects both development and antibiotic production in Streptomyces coelicolor. Its expression is enhanced during the transition to stationary phase. Strains that over-expressed the scr3559 gene region exhibited a shortened exponential growth phase in comparison with a control strain; accelerated aerial mycelium formation and spore maturation; alongside an elevated production of actinorhodin and undecylprodigiosin. These observations were supported by LC-MS analyses of other produced metabolites, including: germicidins, desferrioxamines, and coelimycin. A subsequent microarray differential analysis revealed increased expression of genes associated with the described morphological and physiological changes. Structural and functional similarities between the scr3559 transcript and 6S RNA, and its possible employment in regulating secondary metabolite production are discussed.

Ms1 RNA Interacts With the RNA Polymerase Core in Streptomyces coelicolor and Was Identified in Majority of Actinobacteria Using a Linguistic Gene Synteny Search

“…In Streptomyces coelicolor , positions of the previously published sRNAs are also included. The position of 6S-Like scr3559 was adopted from the 6S-Like scr3559 sequence reported in Figure 2B of Bobek et al (2021) . The position of ssrS /6S RNA was adopted from Mikulík et al (2014) , from the sequences of primers that were used to generate DNA template carrying T7 promoter for in vitro transcription of 6S RNA.…”

“…Recently, ( Bobek et al, 2021 ) reported a new 6S RNA in S. coelicolor , which was named “6S-like scr3559 RNA.” It is expressed from the sco3558-sco3559 genomic locus but from the minus strand (the previously published 6S RNA sequence was expressed from the plus strand), with its transcription start site located at position 3,934,888 in the genome ( Bobek et al, 2021 ). This 6S-like RNA is processed and its 5′ end corresponds to position 3,934,820 ( Bobek et al, 2021 ). However, the processed 6S-like RNA was almost undetectable in wild type S. coelicolor by Northern blotting ( Bobek et al, 2021 ).…”

“…This 6S-like RNA is processed and its 5′ end corresponds to position 3,934,820 ( Bobek et al, 2021 ). However, the processed 6S-like RNA was almost undetectable in wild type S. coelicolor by Northern blotting ( Bobek et al, 2021 ). In addition, the 6S-like RNA does not correspond to the scr3559 sRNA that is transcribed from the plus strand with the transcription start site at position 3,934,693 ( Moody et al, 2013 ; Jeong et al, 2016 ).…”

“…In addition, the 6S-like RNA does not correspond to the scr3559 sRNA that is transcribed from the plus strand with the transcription start site at position 3,934,693 ( Moody et al, 2013 ; Jeong et al, 2016 ). Both sequences of the putative 6S RNAs ( Mikulík et al, 2014 ; Bobek et al, 2021 ) differ from scr3559, although scr3559 is the main transcript derived from the sco3558-sco3559 intergenic region as detected by RNA-seq ( Vockenhuber et al, 2011 ; Moody et al, 2013 ). The function of scr3559 is unknown.…”

Inhibitory potentials of Streptomyces exfoliatus strain ‘MUJA10’ against bacterial pathogens isolated from rural areas in Riyadh, Saudi Arabia

“…These are found in soils, manure, and other sources [ 18 , 19 ]. These are eubacteria and grow by forming filaments or mycelium and do not form the usual bacterial assembly as bacillary or coccoid forms [ 20 ]. They produce chained conidiospores from spore-bearing aerial hyphae.…”