Untitled

Boukhalfa, Hakim; Crumbliss, Alvin L.

doi:10.1023/a:1020218608266

Cited by 482 publications

(257 citation statements)

References 62 publications

Supporting

Mentioning

250

Contrasting

Unclassified

Order By: Relevance

“…However, significant differences were found in both SORI CAD and ECD of metal complexes, dependent on the nature of the metal ion. Intriguingly, unique behavior, consistent with a recently proposed solution-phase structure, was observed for the highly preferred iderophores are low molecular weight, highaffinity iron-chelating agents produced by bacteria, fungi, and marine organisms to solubilize and scavenge Fe 3ϩ from the environment [1][2][3][4]. A wide structural versatility has been found in the more than 200 siderophores reported so far [4].…”

supporting

confidence: 75%

Collision-activated dissociation, infrared multiphoton dissociation, and electron capture dissociation of the Bacillus anthracis siderophore petrobactin and its metal ion complexes

Liu

Håkansson

Lee

et al. 2007

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Siderophores are high-affinity iron-chelating ligands produced by microorganisms to scavenge vital Fe 3ϩ from the environment. Thus, siderophores constitute potential therapeutic targets and their structural determination is important for exploiting their therapeutic value. Here, the virulence-associated siderophore petrobactin from Bacillus anthracis was characterized with electron capture dissociation (ECD). Fragmentation of doubly protonated petrobactin was investigated and compared to sustained off-resonance irradiation collision-activated dissociation (SORI CAD) and infrared multiphoton dissociation (IRMPD) of both the singly and doubly protonated species. These experiments demonstrate that ECD provides additional information (complementary bond cleavages) on the structure of petrobactin compared to both SORI CAD and IRMPD. Furthermore, complexes of petrobactin with divalent (Ca , and Co 2ϩ ) and trivalent (Fe 3ϩ and Ga 3ϩ ) metal cations were also subjected to SORI CAD and ECD. Again, most structural information was obtained from the ECD spectra. However, significant differences were found in both SORI CAD and ECD of metal complexes, dependent on the nature of the metal ion. Intriguingly, unique behavior, consistent with a recently proposed solution-phase structure, was observed for the highly preferred Fe 3ϩ -petrobactin complex. (J Am Soc Mass Spectrom 2007, 18, 842-849)

show abstract

supporting

confidence: 75%

Collision-activated dissociation, infrared multiphoton dissociation, and electron capture dissociation of the Bacillus anthracis siderophore petrobactin and its metal ion complexes

Liu

Håkansson

Lee

et al. 2007

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…S1) this convention results in change in the priority rules causing opposite assignment for Ru (2). Hence the relative configuration of complex 1 is S* C(1) ,R* Ru (1) ,R* Ru (2) while that of complex 2 is S* C(1) ,R* Ru (1) ,S* Ru (2) .…”

Section: Solid State Structuresmentioning

confidence: 99%

“…Fe 3+ ) in microorganisms. [1][2][3] Hydroxamates are also considered as effective inhibitors of various metalloenzymes. [3][4] Both type of biological activity is obviously connected to their capability of forming stable five-membered [O,O] chelate(s) with various, mostly hard type metal ions.…”

Section: Introductionmentioning

confidence: 99%

[(η6-p-cymene)Ru(H2O)3]2+ binding capability of aminohydroxamates — A solution and solid state study

Parajdi-Losonczi

Bényei

Kováts

et al. 2016

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

show abstract

“…From these data, K′ eff = 1.7 × 10 16 M −1 was calculated for equilibrium reaction 9 at pH 6.5, 50 mM MES, in 200 mM KCl at 20°C. This represents a significant affinity for Fe 3+ , but is the lowest iron affinity of any FeFbpA-X assembly studied to date.23-25 (9) The redox potential of FeFbpA-SO 4 was determined by spectroelectrochemistry using an anaerobic OTTLE cell and methyl viologen as a mediator. Well behaved Nernst plots were obtained ( Figure 3) with a slope corresponding to a single electron transfer and intercept E 1/2 = −158 mV, corresponding to reaction 10 uncorrected for any Fe 2+ (10) dissociation.…”

Section: Sulfate Anion Binding To Apo-fbpamentioning

confidence: 99%

Sulfate as a Synergistic Anion Facilitating Iron Binding by the Bacterial Transferrin FbpA: The Origins and Effects of Anion Promiscuity

et al. 2007

View full text Add to dashboard Cite

The ferric binding protein, FbpA, has been demonstrated to facilitate the transport of naked Fe 3+ across the periplasmic space of several gram-negative bacteria. The sequestration of iron by FbpA is facilitated by the presence of a synergistic anion, such as phosphate or sulfate. Here we report the sequestration of Fe 3+ by FbpA in the presence of sulfate, at an assumed periplasmic pH of 6.5 to form FeFbpA-SO 4 with K′ eff = 1.7 × 10 16 M −1 (at 20°C, 50 mM MES, 200 mM KCl). The iron affinity of the FeFbpA-SO 4 protein assembly is two orders of magnitude lower than when bound with phosphate and is the lowest of any of the FeFbpA-X assemblies yet reported. Iron reduction at the cytosolic membrane receptor may be an essential aspect of the periplasmic iron transport process and with an E 1/2 of −158 mV (NHE), FeFbpA-SO 4 is the most easily reduced of all FeFbpA-X assemblies yet studied. The variation of FeFbpA-X assembly stability (K′ eff ) and ease of reduction (E 1/2 ) with differing synergistic anions X n− are correlated over a range of 14 kJ, suggesting that the variations in redox potentials are due to stabilization of Fe 3+ in FeFbpA-X by X n− . Anion promiscuity of FbpA in the diverse composition of the periplasmic space is illustrated by the ex vivo exchange kinetics of FeFbpA-SO 4 with phosphate and arsenate, with first order kinetics with respect to FeFbpA-SO 4 (k = 30 s −1 ) at pH 6.5, independent of entering anion concentration and identity. Anion lability and influence on the iron affinity and reduction potential for FeFbpA-X support the hypothesis that synergistic anion exchange may be an important regulator in iron delivery to the cytosol. This structural and thermodynamic analysis of anion binding in FeFbpA-X provides additional insight into anion promiscuity and importance.

show abstract

Untitled

Cited by 482 publications

References 62 publications

Collision-activated dissociation, infrared multiphoton dissociation, and electron capture dissociation of the Bacillus anthracis siderophore petrobactin and its metal ion complexes

Collision-activated dissociation, infrared multiphoton dissociation, and electron capture dissociation of the Bacillus anthracis siderophore petrobactin and its metal ion complexes

[(η6-p-cymene)Ru(H2O)3]2+ binding capability of aminohydroxamates — A solution and solid state study

Sulfate as a Synergistic Anion Facilitating Iron Binding by the Bacterial Transferrin FbpA: The Origins and Effects of Anion Promiscuity

Contact Info

Product

Resources

About