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Ogbonna, James C.; Soejima, Toshihiko; Ugwu, Charles U.; Tanaka, Hideo

doi:10.1023/a:1011693606473

Cited by 8 publications

(1 citation statement)

References 12 publications

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“…50 mL samples from the outdoor culture were centrifuged at 3000 rpm for 10 min (Juan C142 benchtop centrifuge), washed with deionised water and the biomass added to 300 mL of autoclaved Zarrouk medium contained in a 500 mL Schott™ flask, to produce an initial yield of 1.45 ± 0.10 gL −1 . The relatively high biomass concentration was to minimise potential biological contamination as described by Ogbonna et al [ 38 ]. No acclimatisation of the biomass to indoor, low light conditions, etc.…”

Section: Methodsmentioning

confidence: 99%

Co-Producing Phycocyanin and Bioplastic in Arthrospira platensis Using Carbon-Rich Wastewater

Shayesteh

Laird

Hughes

et al. 2023

BioTech

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Microalgae can treat waste streams containing elevated levels of organic carbon and nitrogen. This process can be economically attractive if high value products are created simultaneously from the relatively low-cost waste stream. Co-production of two high value microalgal products, phycocyanin and polyhydroxybutyrate (PHB), was investigated using non-axenic Arthrospira platensis MUR126 and supplemental organic carbon (acetate, oxalate, glycerol and combinations). All supplemented cultures had higher biomass yield (g/L) than photoautotrophic control. All cultures produced PHB (3.6–7.8% w/w), except the control and those fed oxalate. Supplemented cultures showed a two to three-fold increase in phycocyanin content over the eight-day cultivation. Results indicate co-production of phycocyanin and PHB is possible in A. platensis, using mixed-waste organic carbon. However, supplementation resulted in growth of extremophile bacteria, particularly in cultures fed glycerol, and this had a negative impact on culture health. Refinement of the carbon dosing rate is required to minimise impacts of native bacterial contamination.

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Section: Methodsmentioning

confidence: 99%

Co-Producing Phycocyanin and Bioplastic in Arthrospira platensis Using Carbon-Rich Wastewater

Shayesteh

Laird

Hughes

et al. 2023

BioTech

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Culture Systems Incorporating Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae

Ogbonna

McHenry

2015

Biofuel and Biorefinery Technologies

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The feasibility of using various culture systems incorporating heterotrophic metabolism for biodiesel oil production was compared. Heterotrophic culture can be used to achieve high cell concentration, and depending on the strain and organic carbon source employed, the introduction of light (mixotrophic culture) can enhance cell growth and oil accumulation. However, mixotrophic cultures also face the problem of light limitation, and depending on the relative concentrations of the organic carbon source and light intensity, the interaction between the heterotrophic and photoautotrophic metabolic activities can have negative effects on cell growth and oil accumulation. Systems that separate the two metabolic activities in time or space, such as cyclic photoautotrophic-heterotrophic cultures, sequential heterotrophic-photoautotrophic cultures, and sequential photoautotrophic-mixotrophic cultures, can all be used to improve oil productivity. However, the effectiveness of each system depends on the strain of microalgae and other culture conditions.

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