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Nieves, Nadina; Martínez, M. E.; Castillo, R; Blanco, M.; González-Olmedo, J. L.

doi:10.1023/a:1010699532641

Cited by 35 publications

(4 citation statements)

References 25 publications

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“…The most effective pre-treatment is indicated by * Pretreatment with ABA has also been shown to increase the survival of desiccated encapsulated somatic embryos of sugarcane (Nieves et al 2001) and in a recent study Suzuki et al (2006) demonstrated that ABA and proline are important for both desiccation and cryopreservation tolerance of Gentian axillary buds. Although the importance of ABA in protecting plants against desiccation and low temperatures is well established (Nieves et al 2001) little is known about the role of proline. Recent research suggests that as well as functioning as a cellular osmoticant and membrane protectant, proline can react with some reactive oxygen species (ROS) potentially reducing oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

Efficient cryopreservation of adventitious shoots of Begonia x erythrophylla using encapsulation–dehydration requires pretreatment with both ABA and proline

Burritt

2008

Plant Cell Tiss Organ Cult

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Despite the widespread use of tissue culture as a means of propagating begonias and concerns regarding the preservation of germplasm, little information is available on the cryopreservation of these commercially important plants. For this reason studies were conducted to develop an encapsulation-dehydration method for the cryopreservation of adventitious shoots of the rhizomatous begonia, Begonia x erythrophylla. Adventitious shoots of B. x erythrophylla were found to be sensitive to dehydration and very sensitive to freezing. While pretreatment with 0.75 M sucrose significantly increased the percentage of encapsulated shoots surviving dehydration, pre-treatment with sucrose did not afford cryoprotection without prior dehydration. Addition of abscisic acid and proline to the pretreatment medium significantly improved the percentage of shoots surviving freezing. Pre-treatment of shoots with a medium containing, 0.75 M sucrose, 3.8 lM abscisic acid and 2.15 mM proline resulted in greater than 50% of shoots surviving freezing.

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Section: Resultsmentioning

confidence: 99%

Efficient cryopreservation of adventitious shoots of Begonia x erythrophylla using encapsulation–dehydration requires pretreatment with both ABA and proline

Burritt

2008

Plant Cell Tiss Organ Cult

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“…The results showed that auxin response factor ( ARF ) [11,13,14], leafy cotyledon ( LEC ) [10,15], Wuschel ( WUS ) [16,17,18], somatic embryogenesis receptor kinase ( SERK ) [8], shoot meristemless ( STM ), and baby boom ( BBM ) [10,19,20] were involved in SE. In addition, the salicylic acid (SA) and jasmonic acid (JA) signaling pathways were also predicted to regulate SE [12,21,22,23,24,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton

Guo

Zhang

et al. 2019

IJMS

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Plant regeneration via somatic embryogenesis (SE) is a key step during genetic engineering. In the current study, integrated widely targeted metabolomics and RNA sequencing were performed to investigate the dynamic metabolic and transcriptional profiling of cotton SE. Our data revealed that a total of 581 metabolites were present in nonembryogenic staged calli (NEC), primary embryogenic calli (PEC), and initiation staged globular embryos (GE). Of the differentially accumulated metabolites (DAMs), nucleotides, and lipids were specifically accumulated during embryogenic differentiation, whereas flavones and hydroxycinnamoyl derivatives were accumulated during somatic embryo development. Additionally, metabolites related to purine metabolism were significantly enriched in PEC vs. NEC, whereas in GE vs. PEC, DAMs were remarkably associated with flavonoid biosynthesis. An association analysis of the metabolome and transcriptome data indicated that purine metabolism and flavonoid biosynthesis were co-mapped based on the Kyoto encyclopedia of genes and genomes (KEGG) database. Moreover, purine metabolism-related genes associated with signal recognition, transcription, stress, and lipid binding were significantly upregulated. Moreover, several classic somatic embryogenesis (SE) genes were highly correlated with their corresponding metabolites that were involved in purine metabolism and flavonoid biosynthesis. The current study identified a series of potential metabolites and corresponding genes responsible for SE transdifferentiation, which provides a valuable foundation for a deeper understanding of the regulatory mechanisms underlying cell totipotency at the molecular and biochemical levels.

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“…Polyamines are significantly important in the regulation of cell division, protein synthesis, and other cellular processes in leaves (Nieves et al, 1998;Alcaźar et al, 2010). The availability of N influences the cellular PA biosynthesis in plants.…”

Section: Discussionmentioning

confidence: 99%

Polyamine Metabolism, Photorespiration, and Excitation Energy Allocation in Photosystem II Are Potentially Regulatory Hubs in Poplar Adaptation to Soil Nitrogen Availability

Siddiqui

et al. 2020

Front. Plant Sci.

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Nitrogen fertilization is common for poplar trees to improve growth and productivity. The utilization of N by poplar largely depends on fertilizer application patterns; however, the underlying regulatory hubs are not fully understood. In this study, N utilization and potentially physiological regulations of two poplar clones (XQH and BC5) were assessed through two related experiments (i: five levels of N supply and ii: conventional and exponential N additions). Poplar growth (leaf area) and N utilization significantly increased under fertilized compared to unfertilized conditions, whereas photosynthetic N utilization efficiency significantly decreased under low N supplies. Growth characteristics were better in the XQH than in the BC5 clone under the same N supplies, indicating higher N utilization efficiency. Leaf absorbed light energy, and thermal dissipation fraction was significantly different for XQH clone between conventional and exponential N additions. Leaf concentrations of putrescine (Put) and acetylated Put were significantly higher in exponential than in conventional N addition. Photorespiration significantly increased in leaves of XQH clone under exponential compared to conventional N addition. Our results indicate that an interaction of the clone and N supply pattern significantly occurs in poplar growth; leaf expansion and the storage N allocations are the central hubs in the regulation of poplar N utilization.

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Cited by 35 publications

References 25 publications

Efficient cryopreservation of adventitious shoots of Begonia x erythrophylla using encapsulation–dehydration requires pretreatment with both ABA and proline

Efficient cryopreservation of adventitious shoots of Begonia x erythrophylla using encapsulation–dehydration requires pretreatment with both ABA and proline

Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton

Polyamine Metabolism, Photorespiration, and Excitation Energy Allocation in Photosystem II Are Potentially Regulatory Hubs in Poplar Adaptation to Soil Nitrogen Availability

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