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Behavior of PQQ Glucose Dehydrogenase on Prussian Blue‐Modified Carbon Electrode

“…34-1 (GDH) was purified in the Institute of Biochemistry (Lithuania) [28]. The enzyme stock solution had an activity of 64 U · mL À1 and a concentration of 20 mg protein mL…”

Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications

“…33, E.C. 1.1.5.5, was isolated and purified by the method reported in [51]. The activity of the 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 7 enzyme solution in 5 mM Tris buffer (pH 7.5) with 1 mM CaCl 2 , 0.02 % Triton-X-100 and 0.5% sucrose was 200 U/ml.…”

Bioelectrocatalysis of Pyruvate with PQQ-dependent Pyruvate Dehydrogenase

“…33 (DSM 3504) and Gluconobacter sp. 33 (ATCC 15163), were first cultivated aerobically in a basal media containing glucose, yeast extract, and calcium carbonate (GYC) at 30 °C for 24 hours in petri dishes as per the procedure described in Reference (11). Following preliminary studies, the cultivation procedure was changed to using a media consisting of the following (g/L): yeast extract-5, D-mannitol-10, (NH 4 ) 2 HPO 4 -1.0, and MgSO 4 x 7H 2 O-2.0 with the pH adjusted to 5.5.…”

“…In-situ hydroxyapatite purification step, along with the first of a series of ion exchange column procedures (DEAE-Toyo Pearl ® and CM-Toyo Pearl ® ), are the first steps in the The following literature sources for PQQ-dependent alcohol dehydrogenase purification from Gluconobacter were used as a guideline for the ion exchange chromatography purification scheme (11,12). After removing an insoluble precipitate by centrifugation, the enzyme extract was applied to the DEAE Toyo-Pearl 650M ® anion exchange column (2.5 x 25 cm) which was equilibrated with the dialysis buffer.…”

“…Active fractions collected from the CM-Toyo-Pearl cation exchange column were concentrated and applied to a Sephadex G-100 size exclusion column (1.5 cm x 10 cm) equilibrated with 5 mM potassium phosphate buffer pH 7.2 containing 1mM CaCl 2 and 50 mM NaCl. Fractions were eluted using the same buffer and were also rose colored similar to other purified PQQ-containing enzymes discussed in literature (11,12). , 25 (28) 1-11 (2010) DET at Gold Electrodes Modified with Adsorbed Enzyme Gold electrodes (2mm diameter) were prepared prior to modification by polishing on a Buehler polishing cloth with 0.05micron alumina followed by a methanol rinse and rinsing with 18 MΩ water to insure the electrode surface is uniformly clean and there is no prior fouling.…”